Biolegend, 138703, PE anti-mouse Ly-49A, 25μg

Ly-49A is a 44 kD member of the C-type lectin/Ly-49 family proteins. It is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains. Ly-49A is present on a subset of NK and NK-T cells. Ly-49A, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. The Ly-49A receptor recognizes H-2D d and H-2D k , triggering phosphorylation of the ITIM motif in the cytoplasmic domain and recruitment of SHP-1 and SHP-2. The A1/Ly49A antibody preferentially reacts with CRD of Ly-49A B6 alloantigen and has also shown cross-reaction with Ly-49A NOD , Ly-49P NOD and Ly-49P 129/J .
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse C57BL/6N T lymphoma EL-4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1.
Application References(PubMed link indicates BioLegend citation): Nagasawa R, et al. 1987. J. Immunol. 138:815. (IP)Guy-Grand D, et al. 1996. Eur. J. Immunol. 26:2248. (FC)Zimmer J, et al. 2001. J. Exp. Med. 194:1531. (FC)
Product Citations: Wagner JA, et al. 2020. Cell Rep. 31:107720. PubMed Kissiov DU, et al. 2022. Elife. 11:. PubMed
RRID: AB_2134787 (BioLegend Cat. No. 138703)
Structure: C-type lectin, Ly-49 family, ITIM, 44 kD
Distribution: NK cells, NKT cells
Function: Inhibitory NK cell receptor
Ligand/Receptor: H-2Dd, H-2Dk, H-2Dp
Cell Type: NK cells, NKT cells
Biology Area: Immunology
Molecular Family: MHC Antigens
Antigen References: 1. Coles MC, et al. 2000. Eur. J. Immunol. 30:236. 2. Takei FJ, et al. 1997. Immunol. Rev. 155:67. 3. Hanke T, et al. 1999. Immunity 11:67. 4. Silver ET, et al. 2000. J. Immunol. 165:1771.
Gene ID: 16627
UniProt: View information about Ly-49AB6 on UniProt.org
Clone: A1/Ly49A
Regulatory Status: RUO
Other Names: Ly49A
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.