Biolegend, 139105, Alexa Fluor® 647 anti-mouse CD272 (BTLA) Antibody, 25μg

CD272, also known as B and T lymphocyte attenuator (BTLA), is an Ig superfamily co-inhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4.  BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand herpes virus entry mediator (HVEM) is critical for negatively regulating immune response.  The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8 + memory T cell population, increased severity of colitis, and reduced effectiveness of T regulatory cells.  BTLA plays an important role in the induction of peripheral tolerance of both CD4 + and CD8 + T cells in vivo .  Tolerant T cells have significantly higher expression of BTLA compared with effectors and naïve T cells.  BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity.  It was reported that BTLA may regulate T cell function by binding to B7-H4, but further studies are needed to confirm.  The existence of three distinct BTLA alleles has been reported.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: C57BL/6 BTLA Ig domain protein in CFA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for relevant formats) include: immunoprecipitation1,2 and blocking3,5 of OT-I T cell responses in vitro and incidence of cerebral malaria in vivo caused by P. berghei ANKA (PbA) infection. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 139104). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 139117 - 139122) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg). Clone 6A6 antibody reacts only with the C57BL/6 allele of BTLA but not the BALB/c allele of BTLA.
Application References(PubMed link indicates BioLegend citation): Hurchla MA, et al. 2005. J. Immunol. 174:337. (IP FC) Sedy JR, et al. 2005. Nat. Immunol. 6:90. (IP) Lepenies B, et al. 2007. J. Immunol. 179:4093. (Block) Tao R, et al. 2005. J. Immunol. 175:5774. (FC) Albring J, et al. 2010. J. Exp. Med. 207:2551. (Block)
Product Citations: Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
RRID: AB_10613297 (BioLegend Cat. No. 139106)
Structure: An Ig superfamily co-inhibitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4.
Distribution: BTLA is expressed on a wide number of lymphocytes in mice. It is most highly expressed on B cells, followed by CD4+ T cells, lower expression on CD8+ T cells, macrophages, dendritic cells, NKT cells, and NK cells.
Function: BTLA functions as a negative regulator of T cell activation and proliferation, attenuate B cell proliferation upon associating with its known ligand, herpes virus entry mediator (HVEM).
Ligand/Receptor: HVEM.
Cell Type: B cells, Dendritic cells, Lymphocytes, NKT cells, T cells
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Liu X, et al. 2009. J Immunol. 182:4516. 2. Miller ML, et al. 2009. J Immunol. 183:32. 3. Sun Y, et al. 2009. J Immunol. 183:1946. 4. Vendel AC, et al. 2009. J Immunol. 182:1509. 5. Watanabe N, et al. 2003. Nat Immunol. 4:670.
Gene ID: 208154
UniProt: View information about CD272 on UniProt.org
Clone: 6A6
Regulatory Status: RUO
Other Names: BTLA, B and T lymphocyte attenuator
Isotype: Armenian Hamster IgG