Biolegend, 139301, Purified anti-mouse CD64 (FcγRI) Antibody, 50μg

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.
Application References(PubMed link indicates BioLegend citation): Tan PS, et al. 2003. J. Immunol. 170:2549. (IP) Ingersoll MA, et al. 2010. Blood 115:e10. (FC) Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Rosina M, et al. 2022. Cell Metab. 34:533. PubMed Blériot C, et al. 2020. Methods Mol Biol. 2164:87. PubMed Malinczak CA, et al. 2021. J Immunol. 206:1315. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed Melo GD, et al. 2017. Sci Rep. 6.1625. PubMed Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed Arimura K, et al. 2017. Mucosal Immunol. 1.08125. PubMed Orecchioni M, et al. 2022. Methods Mol Biol. 2419:779. PubMed Rissiek B, et al. 2017. Sci Rep. . 10.1038/s41598-017-16613-w. PubMed Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed Yan J, et al. 2020. Cancer Immunol Res. 8:356. PubMed Feuerstein R, et al. 2020. Elife. 9:00. PubMed Barvalia M, et al. 2022. Methods Mol Biol. 2508:147. PubMed Ozeri E, et al. 2012. J Immunol. 189:146. PubMed Lim PJ, et al. 2019. Nat Metab. 0.402083333. PubMed Blriot C, et al. 2021. Immunity. :. PubMed Janela B, et al. 2019. Immunity. 50:1069. PubMed Gubin MM, et al. 2018. Cell. 175:1014. PubMed Galbas T, et al. 2017. J Immunol. 198(2):852-861. PubMed Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed Qiu Z, et al. 2022. Cancer Discov. 12:502. PubMed Gong P, et al. 2022. Cell Death Discov. 8:466. PubMed Diao J, et al. 2018. J Immunol. 201:1306. PubMed Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed Richardson M, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
RRID: AB_10612757 (BioLegend Cat. No. 139301) AB_10613107 (BioLegend Cat. No. 139302)
Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, mast cells, dendritic cells
Function: Phagocytosis, ADCC
Ligand/Receptor: IgG
Cell Type: Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Gene ID: 14129
UniProt: View information about CD64 on UniProt.org
Clone: X54-5/7.1
Regulatory Status: RUO
Other Names: FcRI
Isotype: Mouse IgG1, κ