Biolegend, 139305, APC anti-mouse CD64 (FcγRI) Antibody, 25μg

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.
Application References(PubMed link indicates BioLegend citation): Tan PS, et al. 2003. J. Immunol. 170:2549. (IP) Ingersoll MA, et al. 2010. Blood 115:e10. (FC) Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Yu‐Han Chang et al. 2017. Immunity. 47(5):943-958 . PubMed Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Chen R, et al. 2023. Acta Pharm Sin B. 13:128. PubMed Cheong LY, et al. 2023. Nat Commun. 14:1213. PubMed Sun JY, et al. 2023. Sci Adv. 9:eade4110. PubMed Coelho DR, et al. 2023. iScience. 26:106442. PubMed Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed Brog RA, et al. 2022. Cancer Immunol Res. 10:962. PubMed Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed Choi H, et al. 2023. J Nanobiotechnology. 21:5. PubMed Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed Bhattacharyya A, et al. 2022. Am J Physiol Lung Cell Mol Physiol. 322:L495. PubMed Jameson VJ, et al. 2022. Cytometry A. 101:922. PubMed Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed Wang KC, et al. 2018. Immunohorizons. 2:407. PubMed de Tymowski C et al. 2019. Cell reports. 27(3):762-775 . PubMed Kaur K, et al. 2021. Cell Rep. 37:110178. PubMed Earnest JT, et al. 2021. Cell Reports. 35(1):108962. PubMed Rothchild AC, et al. 2020. Bio Protoc. 10:00. PubMed Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed Maisonneuve C, et al. 2021. Cell Reports. 34(4):108677. PubMed Qi X, et al. 2019. Nat Commun. 10:2141. PubMed Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed Galle E, et al. 2022. Genome Biol. 23:207. PubMed Marangoni F, et al. 2021. Cell. . PubMed Privratsky JR, et al. 2018. Am J Physiol Renal Physiol. 315:F682. PubMed Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed Qayum A, et al. 2016. J Immunol. 196: 4457 - 4467. PubMed Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed Zhang S, et al. 2019. Mol Med Rep. 19:5377. PubMed Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed Holly Bachus et al. 2018. Immunity. 50(1):225-240 . PubMed Yang J, et al. 2022. Commun Biol. 5:124. PubMed Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed Zhou Q, et al. 2014. J Immunol. 193:496. PubMed Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed Martin T, et al. 2018. Arch Toxicol. 92:323. PubMed Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed Steuerman Y et al. 2018. Cell systems. 6(6):679-691 . PubMed Qian J, et al. 2021. Int J Mol Sci. 22:. PubMed Monaghan KL, et al. 2020. J Vis Exp. . PubMed Meliopoulos V, et al. 2016. PLoS Pathog. 12: 1005804. PubMed Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed Terhorst D, et al. 2015. J Immunol. 195: 4953 - 4961. PubMed Clark JT, et al. 2021. eLife. 10:00. PubMed Stacy A, et al. 2021. Cell. 184(3):615-627.e17. PubMed Gubin MM, et al. 2018. Cell. 175:1014. PubMed Camara A, et al. 2019. Immunity. 50:1467. PubMed Spix B, et al. 2022. Nat Commun. 13:318. PubMed Andriessen EMMA, et al. 2021. EMBO Mol Med. 13:e11754. PubMed Morikawa M, et al. 2016. PLoS One. 11:e0163607. PubMed Sencio V, et al. 2020. Cell Reports. 30(9):2934-2947. PubMed Zwick M, et al. 2019. Front Immunol. 10:222. PubMed Penke LR, et al. 2020. Life Sci Alliance. 3:. PubMed Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed Barbet G, et al. 2018. Immunity. 48:584. PubMed Ntokou A, et al. 2021. JCI Insight. 6:. PubMed Wong NR, et al. 2021. Immunity. 54:2072. PubMed Montalban-Arques A, et al. 2021. Cell Host Microbe. :. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Baratin M, et al. 2017. Immunity. 47:349. PubMed Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed Markworth JF, et al. 2020. JCI Insight. 5:00. PubMed Molgora M, et al. 2020. Cell. 182:886. PubMed Mujal AM, et al. 2022. Cancer Immunol Res. 10:403. PubMed Gong P, et al. 2022. Cell Death Discov. 8:466. PubMed Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed Silva HM, et al. 2019. J Exp Med. 216:786. PubMed Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed Rochford I, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321:L686. PubMed Joshi JC, et al. 2020. Cell Rep. 30:4096. PubMed Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed Rivera CA, et al. 2022. Immunity. 55:129. PubMed Boddupalli CS, et al. 2022. Elife. 11:. PubMed Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed Huang WC, et al. 2019. Int J Hyperthermia. 36:1255. PubMed Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed Deets KA, et al. 2021. Elife. 10:. PubMed
RRID: AB_11219205 (BioLegend Cat. No. 139305) AB_11219391 (BioLegend Cat. No. 139306)
Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, mast cells, dendritic cells
Function: Phagocytosis, ADCC
Ligand/Receptor: IgG
Cell Type: Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Gene ID: 14129
UniProt: View information about CD64 on UniProt.org
Clone: X54-5/7.1
Regulatory Status: RUO
Other Names: FcRI
Isotype: Mouse IgG1, κ