Biolegend, 139311, Brilliant Violet 711™ anti-mouse CD64 (FcγRI) Antibody, 50μg

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.
Application References(PubMed link indicates BioLegend citation): Tan PS, et al. 2003. J. Immunol. 170:2549. (IP) Ingersoll MA, et al. 2010. Blood 115:e10. (FC) Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed Yu‐Han Chang et al. 2017. Immunity. 47(5):943-958 . PubMed Solier S, et al. 2023. Int J Mol Sci. 24:. PubMed Harpur CM, et al. 2023. Clin Transl Immunology. 12:e1443. PubMed Rosina M, et al. 2022. Cell Metab. 34:533. PubMed West HC, et al. 2022. Cell Rep. 39:110819. PubMed Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed Siret C, et al. 2022. Nat Commun. 13:7366. PubMed Wang KC, et al. 2018. Immunohorizons. 2:407. PubMed Shmuel-Galia L, et al. 2021. Immunity. 54(6):1137-1153.e8. PubMed Scott CL et al. 2018. Immunity. 49(2):312-325 . PubMed Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed Bauer KM, et al. 2022. JCI Insight. 7:. PubMed Bauché D et al. 2018. Immunity. 49(2):342-352 . PubMed Rao E, et al. 2021. Sci Immunol. 6:. PubMed Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed Di Gioia M, et al. 2020. Nat Immunol. 21:42. PubMed Xu M et al. 2018. Immunity. 48(4):787-798 . PubMed Devos M, et al. 2020. J Exp Med. 217:00:00. PubMed Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed Lin J, et al. 2017. Sci Rep. 7:41722. PubMed Benechet AP, et al. 2019. Nature. 574:200. PubMed Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed Bonnardel J, et al. 2019. Immunity. 51:638. PubMed Cho YK, et al. 2022. Nat Commun. 13:4084. PubMed Yang C, et al. 2021. J Invest Dermatol. 810:141. PubMed Goossens P, et al. 2019. Cell Metab. 29:1376. PubMed Tondini E, et al. 2022. NPJ Vaccines. 7:64. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Prooyen N, et al. 2016. PLoS One. 12: 1005749. PubMed de Reuver R, et al. 2021. Cell Reports. 36(6):109500. PubMed De Simone G, et al. 2021. Immunity. :. PubMed Blriot C, et al. 2021. Immunity. :. PubMed Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed Guilliams M, et al. 2022. Cell. 185:379. PubMed Gentek R, et al. 2018. Immunity. 48:1160. PubMed Etzerodt A, et al. 2019. J Exp Med. 216:2394. PubMed Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed Cordeiro B, et al. 2020. Cell Reports. 31(5):107585. PubMed Van Nuffel E, et al. 2020. EMBO Rep. 21:e49237. PubMed Scheraga RG, et al. 2020. J Immunol. 1310:204. PubMed Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed Mogilenko DA et al. 2019. Cell. 177(5):1201-1216 . PubMed Catrysse L, et al. 2021. Cell Rep. 36:109748. PubMed Filtjens J, et al. 2021. Nat Commun. 12:2936. PubMed Etzerodt A, et al. 2020. J Exp Med. 217:00:00. PubMed Goldberg MF et al. 2018. Immunity. 49(6):1090-1102 . PubMed Buschor S, et al. 2017. PLoS Pathogens. 13(6):e1006476. PubMed
RRID: AB_2563846 (BioLegend Cat. No. 139311)
Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, mast cells, dendritic cells
Function: Phagocytosis, ADCC
Ligand/Receptor: IgG
Cell Type: Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Gene ID: 14129
UniProt: View information about CD64 on UniProt.org
Clone: X54-5/7.1
Regulatory Status: RUO
Other Names: FcRI
Isotype: Mouse IgG1, κ