Biolegend, 139316, FITC anti-mouse CD64 (FcγRI) Antibody, 100μg

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.
Application References(PubMed link indicates BioLegend citation): Tan PS, et al. 2003. J. Immunol. 170:2549. (IP) Ingersoll MA, et al. 2010. Blood 115:e10. (FC) Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Mathur R, et al. 2019. Mucosal Immunol. 12:612. PubMed Sanmarco LM, et al. 2022. Nature. 611:801. PubMed Martin-Martin I, et al. 2022. Cell Rep. 39:110648. PubMed Frederico B, et al. 2022. Dev Cell. 57:1957. PubMed Sun Y, et al. 2023. Cell Mol Life Sci. 80:63. PubMed Zhang R, et al. 2021. Cell Mol Immunol. 18:1222. PubMed Khamissi FZ, et al. 2022. Sci Adv. 8:eabm5900. PubMed Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed Nguyen SL, et al. 2021. Sci Rep. 11:4217. PubMed She L, et al. 2021. JCI Insight. 6:e143509. PubMed An J,et al. 2017. Sci Rep.. 10.1038/s41598-017-13629-0. PubMed Grigoryan L, et al. 2022. NPJ Vaccines. 7:55. PubMed Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Liu J, et al. 2018. Am J Pathol. 188:2786. PubMed Liu SS, et al. 2021. Immunity. :. PubMed Kitamoto S, et al. 2020. Cell. 182(2):447-462.e14. PubMed Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
RRID: AB_2566555 (BioLegend Cat. No. 139315) AB_2566556 (BioLegend Cat. No. 139316)
Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, mast cells, dendritic cells
Function: Phagocytosis, ADCC
Ligand/Receptor: IgG
Cell Type: Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Gene ID: 14129
UniProt: View information about CD64 on UniProt.org
Clone: X54-5/7.1
Regulatory Status: RUO
Other Names: FcRI
Isotype: Mouse IgG1, κ