Biolegend, 139504, PE anti-mouse TCR Vβ5.1, 5.2 Antibody, 100μg

Vβ5.1 and 5.2 T cell receptor (TCR Vβ5.1, 5.2) are variants of TCR β chain that, along with TCR α chain, forms the TCR heterodimer. In association with the CD3 complex, TCR α/β is responsible for antigen recognition in the MHC-Peptide complex and the initiation of T cell-mediated immune responses.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Murine T cell hybridoma 2HB51.8
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Induction of proliferation of Vß5.1+ and Vß5.2+ T cells2, 3 and in vivo depletion of Vß5+ T cells4.
Application References(PubMed link indicates BioLegend citation): Kanagawa O, et al. 1991. J. Immunol. 147:1307. (FC) Kanagawa O, et al. 1992. J. Immunol. 149:9. (Activ) Woodland DL, et al. 1993. J. Exp. Med. 177:433. (Activ) Gelber C, et al. 1992. Cancer Res. 52:6507. (Deplete)
Product Citations: Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed Takeda Y, et al. 2018. Innate Immun. 24:323. PubMed Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed Prasad M, et al. 2021. Front Immunol. 12:644483. PubMed Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed Russler-Germain EV, et al. 2021. Elife. 10:. PubMed Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed Morris AB, et al. 2020. Immunity. 52(1):136-150.e6.. PubMed Wang X, et al. 2022. Elife. 11:. PubMed
RRID: AB_10612761 (BioLegend Cat. No. 139503) AB_10613279 (BioLegend Cat. No. 139504)
Structure: Member of the immunoglobulin superfamily, Vβ5.1and 5.2 are variants of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.
Distribution: Expressed on a subset of TCR αβ+ T cells.
Function: Recognition of peptides presented by the MHC molecules, responsible for T cell mediated immune responses.
Ligand/Receptor: MHC-Peptide complex
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Marrack P, et al. 2008. Annu. Rev. Immunol. 26:171. 2. Sim GK and Augustin AA. 1985. Cell 42:89. 3. Mami-Chouaib F, et al. 2002. Immunol. Rev. 188:114.
Gene ID: 21577
UniProt: View information about TCR Vbeta5.1 5.2 on UniProt.org
Clone: MR9-4
Regulatory Status: RUO
Other Names: Vβ5 T-cell receptor, T cell receptor beta 5
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.