Biolegend, 139606, PE anti-mouse MAIR-IV (CLM-5) Antibody, 100μg

MAIR-IV, also known as LMIR4 or CLM5, is a member of the myeloid-associated immunoglobulin-like receptor family (MAIR, CD300). It is preferentially expressed on Gr-1 + /Mac-1 + neutrophils in the peripheral blood, bone marrow, peritoneal cavity, and spleen. It is also found on Mac-1 + /Gr-1 + macrophages and CD11c + dendritic cells. In association with the immunoreceptor tyrosine based activating motif (ITAM)-bearing adaptor FcRγ, MAIR-IV mediates activation signals for neutrophils.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: MAIR-IV transfected BW5147 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blot1, plate-coated TX69 induced secretion of IL-6, and TNF-α by neutrophils1.
Application References(PubMed link indicates BioLegend citation): Nakano T, et al. 2008. Mol. Immunol. 45:289. (Activ, WB)
Product Citations: Nagano N, et al. 2020. Biomedicines. :8. PubMed Graziano VR, et al. 2020. PLoS Pathog. 16:e1008242. PubMed
RRID: AB_10641143 (BioLegend Cat. No. 139605)
Structure: Ig superfamily, associates with ITAM-bearing adaptor FcRγ
Distribution: Neutrophils, macrophages, dendritic cells
Function: Activates neutrophils
Cell Type: Neutrophils, Macrophages, Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Shibuya A, et al. 2009. Immune Network 9:41. 2. Can I, et al. 2008. J. Immunol. 180:207. 3. Nakano T, et al. 2008. Mol. Immunol. 45:289.
Gene ID: 217305
UniProt: View information about MAIR-IV on UniProt.org
Clone: TX69
Regulatory Status: RUO
Other Names: LMIR4
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.