Biolegend, 139703, PE anti-mouse TCR Vβ12 Antibody, 25μg

T cell receptor Vβ12 (TCR Vβ12) is a variant of TCR β chain and, along with TCR α chain, forms the TCR heterodimer. In association with the CD3 complex, TCR α/β is responsible for antigen recognition through interaction with MHC-peptide complex to initiate the T cell-mediated immune responses. The MR11-1 antibody reacts with the Vβ12 TCR of mice bearing the b haplotype. It has been reported that Mtv-8 and Mtv-9 cause clonally deletion of Vβ12+ T cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Joshi P, et al. 2006. J Leukoc Biol. 80:1345. (FC)
Product Citations: Lui VG, et al. 2024. J Exp Med. :221. PubMed Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed Wang X, et al. 2022. Elife. 11:. PubMed
RRID: AB_10643999 (BioLegend Cat. No. 139703) AB_10639729 (BioLegend Cat. No. 139704)
Structure: Member of the immunoglobulin superfamily, Vβ12 is a variant of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer
Distribution: subset of TCR αβ+ T cells
Function: Recognition of peptides presented in the MHC molecules, responsible for T cell mediated immune responses
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Gene ID: 21577
UniProt: View information about TCR Vbeta12 on UniProt.org
Clone: MR11-1
Regulatory Status: RUO
Other Names: Vβ12 T-cell receptor, T cell receptor beta 12
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.