Biolegend, 139803, PE anti-mouse TCR Vβ9 Antibody, 25μg

Vβ9 T cell receptor (TCR Vβ9) is a variant of TCR β chain and, along with TCR α chain, forms the TCR heterodimer. In association with the CD3 complex, TCR α/β is responsible for antigen recognition through interaction with MHC-peptide complex to initiate T cell-mediated immune responses. Murine Vβ9 cells recognize the Mtv-43, Mtv-44, and exogenous MMTV (SW) Mls-1 superantigens.Mice bearing a and c haplotypes have Vβ9 gene deletion.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse T-cell hybridoma 2BR18
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Utsunomiya Y, et al. 1991. Eur. J. Immunol. 21:1007. (FC)
Product Citations: Prendergast ÁM, et al. 2017. Haematologica. 445:102. PubMed Caeser R, et al. 2019. Nat Commun. 4543:10. PubMed Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
RRID: AB_10640824 (BioLegend Cat. No. 139803) AB_10641563 (BioLegend Cat. No. 139804)
Structure: Member of the immunoglobulin superfamily, Vβ9 is a variant of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.
Distribution: Subset of TCR αβ+ T cells
Function: Recognition of peptides presented by MHC molecules, responsible for T cell mediated immune responses.
Ligand/Receptor: MHC-peptide complex
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Ortman RA, et al. 1994. J. Immunol. 152:4175. 2. Niimi N, et al. 1994. Immunogenetics 40:312. 3. Pied s, et al. 1997. Int. Immunol. 9:17.
Gene ID: 21577
UniProt: View information about TCR Vbeta9 on UniProt.org
Clone: MR10-2
Regulatory Status: RUO
Other Names: Vβ9 T-cell receptor, T cell receptor beta 9
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.