Biolegend, 140005, APC anti-mouse TCR Vβ6 Antibody, 25μg

Vβ6 T cell receptor (TCR Vβ6) is a variant of TCR β chain and, along with TCR α chain, forms the TCR heterodimer. In association with the CD3 complex, TCR α/β is responsible for antigen recognition in the MHC-Peptide complex and the initiation of the T cell-mediated immune responses. Murine Vβ6 cells recognize the Mtv-7, Mls-1, and rabies superantigens.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: H-Y specific TH Clone OI11
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: induction of T cell proliferation1, blocking in vitro cytolysis1, and in vivo blocking of syngeneic T cell tumor growth2.
Application References(PubMed link indicates BioLegend citation): Kanagawa O and Palmer EBJ. 1989. Cell. Immunol. 119:412. (Activ, Block) Kanagawa O. 1989. J. Exp. Med. 170:1513. (Block)
Product Citations: Van NT, et al. 2023. Nat Commun. 7363:14. PubMed Ooi JD, et al. 2017. Nature. 545:243. PubMed George BM, et al. 2020. Cell Stem Cell. 25(2):185-192. PubMed
RRID: AB_2564056 (BioLegend Cat. No. 140005) AB_2564057 (BioLegend Cat. No. 140006)
Structure: Member of the immunoglobulin superfamily, Vβ6 is a variant of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.
Distribution: Expressed on a subset of TCR αβ+ T cells.
Function: Recognition of peptides presented in the MHC molecules, responsible for T cell mediated immune responses.
Ligand/Receptor: MHC-Peptide complex
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Acha-Orbea H and MacDonald HR. 1995. Annu. Rev. Immunol. 13:459. 2. Rudy CK, et al. 1992. J. Exp. Med. 175:1613. 3. Jones LA, et al. 1990. Science 250:1726.
Gene ID: 21577
UniProt: View information about TCR Vbeta6 on UniProt.org
Clone: RR4-7
Regulatory Status: RUO
Other Names: T cell receptor beta 6
Isotype: Rat IgG2b, λ