Biolegend, 140317, Brilliant Violet 605™ anti-mouse CD90.2 (Thy1.2) Antibody, 125microl

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2, a GPI-linked membrane molecule. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45.
125microl
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: µl size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen tissue section, immunofluorescence2, and immunoprecipitation3. Does not react with Thy-1.1 (CD90.1).
Application References(PubMed link indicates BioLegend citation): Aldrich M, et al. 2003. J. Immunol. 171:5562. (IHC) Jameson J, et al. 2004. J. Immunol. 172:3573. (IF) Okada C, et al. 1990. J. Immunol. 144:3473. (IP)
Product Citations: Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed Cautivo KM, et al. 2022. Immunity. 55:254. PubMed Grebinoski S, et al. 2022. Nat Immunol. 23:868. PubMed Chaugule S, et al. 2021. Int J Mol Sci. 22:. PubMed Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed Huang X, et al. 2020. Cell Host Microbe. 29(2):210-221.e6. PubMed Cunningham C, et al. 2016. PLoS Pathog. 12: 1005356. PubMed Trotta E, et al. 2018. Nat Med. 24:1005. PubMed Köchl R, et al. 2020. Elife. 9:00. PubMed Wilmore JR et al. 2018. Cell host & microbe. 23(3):302-311 . PubMed Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed Lin C, et al. 2022. Nat Commun. 13:6869. PubMed de Lucía Finkel P, et al. 2021. Sci Rep. 11:12233. PubMed Lima-Junior DS, et al. 2021. Cell. . PubMed Grauel AL, et al. 2020. Nat Commun. 4.84375. PubMed Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Wilmore JR, et al. 2022. Front Immunol. 12:791095. PubMed Aggio-Bruce R, et al. 2020. Mol Neurobiol. . PubMed Kim JM, et al. 2020. Nat Commun. 11:2289. PubMed Seo GY et al. 2018. Cell host & microbe. 24(2):249-260 . PubMed Wang L, et al. 2020. Nat Commun. 0.654166667. PubMed Han Y, et al. 2019. J Clin Invest. 130:26. PubMed
RRID: AB_11203724 (BioLegend Cat. No. 140317) AB_2650924 (BioLegend Cat. No. 140318)
Structure: Ig superfamily, 25-35 kD
Distribution: Hematopoietic stem cells and neurons, epithelial cells, fibroblasts, neurons, all thymocytes, and peripheral T cells of Thy-1.2 bearing mice
Function: Lymphocyte costimulation, proliferation, and differentiation of hematopoietic stem cells
Ligand/Receptor: CD45
Cell Type: Epithelial cells, Fibroblasts, Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family: CD Molecules
Antigen References: 1. Borrello M, et al. 1996. Cell. Immunol.173:198. 2. Radrizzani M, et al. 1995. J. Neurosci. Res. 42:220. 3. Williams A, et al. 1982. Science 216:696.
Gene ID: 21838
UniProt: View information about CD90.2 on UniProt.org
Clone: 53-2.1
Regulatory Status: RUO
Other Names: Thy-1.2
Isotype: Rat IgG2a, κ