Biolegend, 140321, PerCP/Cyanine5.5 anti-mouse CD90.2 (Thy1.2) Antibody, 25μg

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2, a GPI-linked membrane molecule. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen tissue section, immunofluorescence2, and immunoprecipitation3. Does not react with Thy-1.1 (CD90.1).
Application References(PubMed link indicates BioLegend citation): Aldrich M, et al. 2003. J. Immunol. 171:5562. (IHC) Jameson J, et al. 2004. J. Immunol. 172:3573. (IF) Okada C, et al. 1990. J. Immunol. 144:3473. (IP)
Product Citations: Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed Dragan M, et al. 2022. J Invest Dermatol. 142:583. PubMed Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed Mathur S, et al. 2021. JCI Insight. 6:. PubMed Boothby IC, et al. 2021. Nature. 599:667. PubMed Ku C, et al. 2015. Genes Dev. 29: 1930-1941. PubMed Kolev M, et al. 2020. Immunity. 52(3):513-527. PubMed Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed Wang D, et al. 2022. EMBO Rep. 23:e53691. PubMed Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed
RRID: AB_2562695 (BioLegend Cat. No. 140321) AB_2562696 (BioLegend Cat. No. 140322)
Structure: Ig superfamily, 25-35 kD
Distribution: Hematopoietic stem cells and neurons, epithelial cells, fibroblasts, neurons, all thymocytes, and peripheral T cells of Thy-1.2 bearing mice
Function: Lymphocyte costimulation, proliferation, and differentiation of hematopoietic stem cells
Ligand/Receptor: CD45
Cell Type: Epithelial cells, Fibroblasts, Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family: CD Molecules
Antigen References: 1. Borrello M, et al. 1996. Cell. Immunol.173:198. 2. Radrizzani M, et al. 1995. J. Neurosci. Res. 42:220. 3. Williams A, et al. 1982. Science 216:696.
Gene ID: 21838
UniProt: View information about CD90.2 on UniProt.org
Clone: 53-2.1
Regulatory Status: RUO
Other Names: Thy-1.2
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.