Biolegend, 140413, Pacific Blue™ anti-mouse CD8b.2 Antibody, 25μg

CD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation. The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining4 of frozen tissue section using dry ice-isopentane method and immunoprecipitation5.
Application References(PubMed link indicates BioLegend citation): Ledbetter J, et al. 1979. Immunol. Rev. 47:63. Ledbetter J, et al. 1980. J. Exp. Med. 152:280. Vremec D, et al. 2000. J. Immunol. 164:2978. (IF) Lawrence D, et al. 1999. J. Virol. 73:1795. (IHC) Bosselut R, et al. 1999. J. Exp. Med. 190:1517. (IP)
Product Citations: Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed Lercher A, et al. 2019. Immunity. 51:1074. PubMed Nika L, et al. 2019. Vaccines (Basel). 7. PubMed Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
RRID: AB_10641278 (BioLegend Cat. No. 140414)
Structure: Ig superfamily, CD8β chain, 32 kD
Distribution: Majority of thymocytes, cytotoxic T cell subsets
Function: Co-receptor of TCR
Ligand/Receptor: MHC class I
Cell Type: T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Ledbetter J, et al. 1981. J. Exp. Med. 153:1503. 2. Renard V, et al. 1996. J. Exp. Med. 184:2439.
Gene ID: 12526
UniProt: View information about CD8beta.2 on UniProt.org
Clone: 53-5.8
Regulatory Status: RUO
Other Names: Lyt-3.2, Ly-3.2
Isotype: Rat IgG1, κ