Biolegend, 140704, PE anti-mouse TCR Vβ13 Antibody, 100μg

Vβ13 T cell receptor (TCR Vβ13) is a variant of TCR β chain that along with TCR α chain forms the TCR heterodimer. In association with the CD3 complex, TCR α/β is responsible for antigen recognition in the MHC-Peptide complex and initiation of T cell-mediated immune responses. The most frequently β chains found in the immune response to myelin basic protein (MBP) in B10.PL mice are Vβ13 and Vβ8.2.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vitro blocking of antigen-induced proliferation of Vβ13+ T cells 1 and in vivo depletion of Vβ13+ T cells1.
Application References(PubMed link indicates BioLegend citation): Zaller DM, et al. 1990. J. Exp. Med. 171:1943. (Block)
Product Citations: Vella JL, et al. 2021. Life Sci Alliance. 4:. PubMed
RRID: AB_10645512 (BioLegend Cat. No. 140703) AB_10639945 (BioLegend Cat. No. 140704)
Structure: Member of the immunoglobulin superfamily, Vβ13 is a variant of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.
Distribution: Expressed on a subset of TCR αβ+ T cells.
Function: Recognition of peptides presented by the MHC molecules, responsible for T cell mediated immune responses.
Ligand/Receptor: MHC-Peptide complex
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Zaller DM, et al. 1990. J. Exp. Med. 171:1943. 2. Gentile F, et al. 2004. Immunology 112:13. 3. Lode HN, et al. 1999. P. Natl. Acad. Sci. USA 96:8591.
Gene ID: 21577
UniProt: View information about TCR Vbeta13 on UniProt.org
Clone: MR12-4
Regulatory Status: RUO
Other Names: Vβ13 T-cell receptor, T cell receptor beta 13
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.