Biolegend, 140902, Purified anti-mouse VEGFR-3 (FLT-4) Antibody, 500μg

VEGFR-3, also known as FLT4, is a member of the Tyr protein kinase family. The extracellular portion of VEGFR-3 contains 7 immunoglobulin (Ig)-like domains and the cytoplasmic portion contains a protein kinase domain. FLT4 regulates angiogenesis and lymphangiogenesis, its ligands are VEGF-C and D and its binding is mediated by the 2 nd and 3 rd Ig-like domains of FLT4. During fetal development VEGFR-3 is expressed on endothelial cells, however, in the adult mice, the vascular endothelial cells lose VEGFR-3 expression, but the lymphatic endothelium expresses it constitutively. In addition, VEGFR-3 expression can be induced in tumors with active angiogenesis.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: VEGFR-3-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedBlock, ELISA, IP, IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: ELISA1,2, immunoprecipitation1and Western blot1,2 from cell lysates, immunohistochemical staining1, 3 of frozen and paraffin embedded tissue sections and in whole-mount mouse embryos, in vivo blocking1, 5 of VEGF-C binding to inhibit angiogenesis, lymphangiogenesis, growth and metastasis of different tumor cells, in vitro blocking4 of VEGF-C binding to induce dispersion of endothelial cell clusters.This clone detect multiple bands by western blot: a ~140 kD band corresponding to cleaved/soluble VEGFR-3, and 220 and 230 kD bands corresponding to unglycosylated and glycosylated full length VEGFR-3 (Bando, et al. 2004. Int J Canc. 111:184.)
Additional Product Notes: This product has been reported by a collaborator to work for immunohistochemical staining of frozen tissue sections.
Application References(PubMed link indicates BioLegend citation): Kubo H, et al. 2000. Blood 96:546. (IP, WB, IHC, ELISA, Block) Zhang L, et al. 2010. Cell. Res. Epub ahead of print (WB, ELISA) Tammela T, et al. 2010. Nature 454:656. (IHC) Shimizu K, et al. 2004. Cancer Sci. 95:328. (Block) Matsumura K, et al. 2003. Blood 101:1367. (Block) Lee J, et al. 2012. PNAS 109:19638. PubMed Ozasa R, et al. 2012. J Exp Clin Cancer Res. 31:83. PubMed
Product Citations: Ozasa R, et al. 2012. J Exp Clin Cancer Res. 31:83. PubMed Bieniasz–Krzywiec P, et al. 2020. Cell Metabolism. 30(5):917-936. PubMed Inverso D, et al. 2021. Developmental Cell. 56(11):1677-1693.e10. PubMed Lee J, et al. 2012. Proc Natl Acad Sci U S A. 109:19638. PubMed
RRID: AB_10683463 (BioLegend Cat. No. 140901) AB_10680790 (BioLegend Cat. No. 140902)
Structure: Member of Tyr protein kinase family. Extracellular portion contains 7 immunoglobulin-like domains. Ligand binding is mediated by its domains 2 and 3. Cytoplasmic portion contains 1 protein kinase domain.
Distribution: Expressed on vascular endothelial cells during fetal development, constitutively expressed on lymphatic endothelium in adult mice, induced in tumors with active angiogenesis.
Function: Regulates angiogenesis and lymphangiogenesis.
Interaction: Associated with VEGFR-2 in the surface.
Ligand/Receptor: VEGF-C and D
Cell Type: Endothelial cells
Biology Area: Angiogenesis, Cell Biology, Immunology, Signal Transduction
Molecular Family: Cytokine/Chemokine Receptors, Protein Kinases/Phosphatase
Antigen References: 1. Uhrin P, et al. 2010. Blood 115:3997. 2. Wang Y, et al. 2010. Nature 465:483. 3. Akobsson L, et al. 2009. Biochem. Soc. T. 37:1233. 4. Tammela T, et al. 2010. Nature 454:656. 5. Huang G and Chen L. 2008. Cancer Biother. Radio. 23:661. 6. Zhang YJ and Fang JY. 2008. J. Gastroen. Hepatol. 23:856.
Gene ID: 14257
UniProt: View information about VEGFR-3 on UniProt.org
Clone: AFL4
Regulatory Status: RUO
Other Names: Vascular endothelial growth factor receptor 3, FLT-4, Fms-like tyrosine kinase 4
Isotype: Rat IgG2a, κ