Biolegend, 141213, Brilliant Violet 421™ anti-mouse CD133 Antibody, 50μg

CD133 (also known as prominin-1 and AC133) is a 120 kD pentaspan glycoprotein with 5 transmembrane domains which localize to cellular protrusions. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to hematopoietic stem cells, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. It also has been detected at low levels in the kidney, pancreas, placenta, and fetal liver tissue. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CD133-expressing-GFP-RBL2H3 plus CFA as adjuvant.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Zhang Y, et al. 2012. Diabetes. 61:2114. PubMed
RRID: AB_2566011 (BioLegend Cat. No. 141213)
Structure: 120 kD pentaspan transmembrane glycoprotein
Distribution: Hematopoietic stem cells, epithelial progenitor cells, leukemia cells, brain tumor cells, kidney, pancreas, placenta, and fetal liver cells
Function: Biological function unknown
Cell Type: Epithelial cells, Hematopoietic stem and progenitors, Leukemia, Neural Stem Cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Singh SK, et al. 2004. Nature 18:396. 2. Falini B, et al. 2005. New Engl. J. Med. 352:254. 3. Bonanno G, et al. 2004. Transfusion 44:1087. 4. Gaipa G, et al. 2002. Haematologica 87:347. 5. Wuchter C, et al. 2001. Haematologica 86:154.
Gene ID: 19126
UniProt: View information about CD133 on UniProt.org
Clone: 315-2C11
Regulatory Status: RUO
Other Names: Prominin-1, AC133
Isotype: Rat IgG2a, λ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.