Biolegend, 141405, APC anti-mouse LAP (TGF-β1) Antibody, 25μg

Transforming growth factor beta (TGF-β) is a cytokine that has critical functions in the immune response by regulating Treg and Th17 cells. TGF-β is first synthesized as pro-TGF-β and then it is cleaved by furin proprotein convertase in the Golgi apparatus to produce the dimeric propeptides called latency-associate peptide (LAP) that non-covalently associates with the dimeric mature TGF-β to prevent its activity. This complex can further associate with latent-TGF-β-binding protein (LTBP) to produce a large latent form for deposition onto the extracellular matrix. The latent-TGF-β can be expressed on the membrane of activated Treg cells, immature dendritic cells, megakaryocytes, and platelets.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse Tgfb1-transduced P3U1 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone TW7-16B4 has been reported to not cross-react with bovine LAP.2 Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.2 TW7-16B4 recognizes recombinant LAP, latent TGF-β, and pro-TGF-β. Additional reported applications (for relevant formats) include: Western blotting1 and immunoprecipitation1.
Application References(PubMed link indicates BioLegend citation): Oida T, et al. 2010. PLoS One 5:e15523. (FC, IP, WB) Oida T, et al. 2011. PLoS One 6:e18365. (Neut)
Product Citations: Jayachandran R, et al. 2019. Immunity. 50:152. PubMed Yao G, et al. 2023. Nat Commun. 14:3604. PubMed Lehmkuhl P, et al. 2021. Cell Mol Immunol. 18:1677. PubMed Vick SC, et al. 2021. J Immunol. 207:2598. PubMed Gerlach BD, et al. 2021. Cell Metab. 33:2445. PubMed Suresh V, et al. 2022. FASEB J. 36:e22449. PubMed Li Y, et al. 2022. Brain Behav Immun. 101:1. PubMed Minutti CM, et al. 2019. Immunity. 50:645. PubMed Turner JA, et al. 2020. Immunity. 53:1202. PubMed Kumar SRP, et al. 2020. Front Immunol. 11:844. PubMed Tan X, et al. 2021. Front Oncol. 11:768222. PubMed Gubser C, et al. 2016. Sci Rep. 6: 25758. PubMed Colliou N, et al. 2018. Gut Microbes. 9:279. PubMed Furuya Y, et al. 2015. PLoS Pathog. 11: 1005180. PubMed Mukhopadhyay D, et al. 2015. PLoS Negl Trop Dis. 9: e0004145. PubMed Umeshappa CS, et al. 2021. Cell Reports. 34(13):108919. PubMed Alonso-Herranz L, et al. 2020. Elife. 9:00. PubMed Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed Cai B, et al. 2020. Cell Metabolism. 31(2):406-421.e7.. PubMed Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
RRID: AB_10896418 (BioLegend Cat. No. 141405) AB_10898159 (BioLegend Cat. No. 141406)
Structure: Dimmers of latency-associated peptide non-covalently associated with dimmers of mature TGF-β
Distribution: Many cell types, highly expressed on activated Tregs and platelets
Function: TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response
Ligand/Receptor: TGF-β receptors
Cell Type: Dendritic cells, Platelets, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Oida T, et al. 2010. PLoS One 5:e15523. 2. Tran D, et al. 2009. P. Natl. Acad. Sci. USA 106:13445. 3. Ochi H, et al. 2006. Nat. Med. 12:627. 4. Oida T, et al. 2003. J. Immunol. 170:2516. 5. Nakamura K. 2001. J. Exp. Med. 194:629. 6. Miyazono K, et al. 1993. Growth Factors 8:11.
Gene ID: 21803
UniProt: View information about LAP on UniProt.org
Clone: TW7-16B4
Regulatory Status: RUO
Other Names: Latency Associated Peptide (LAP), Transforming growth factor-beta 1 (TGF-b1), TGFB1, DPD1, TGF-beta1
Isotype: Mouse IgG1, κ