Biolegend, 141407, Brilliant Violet 421™ anti-mouse LAP (TGF-β1) Antibody, 125microl

Transforming growth factor beta (TGF-β) is a cytokine that has critical functions in the immune response by regulating Treg and Th17 cells. TGF-β is first synthesized as pro-TGF-β and then it is cleaved by furin proprotein convertase in the Golgi apparatus to produce the dimeric propeptides called latency-associate peptide (LAP) that non-covalently associates with the dimeric mature TGF-β to prevent its activity. This complex can further associate with latent-TGF-β-binding protein (LTBP) to produce a large latent form for deposition onto the extracellular matrix. The latent-TGF-β can be expressed on the membrane of activated Treg cells, immature dendritic cells, megakaryocytes, and platelets.
125microl
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse Tgfb1-transduced P3U1 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone TW7-16B4 has been reported to not cross-react with bovine LAP.2 Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.2 TW7-16B4 recognizes recombinant LAP, latent TGF-β, and pro-TGF-β. Additional reported applications (for relevant formats) include: Western blotting1 and immunoprecipitation1.
Application References(PubMed link indicates BioLegend citation): Oida T, et al. 2010. PLoS One 5:e15523. (FC, IP, WB) Oida T, et al. 2011. PLoS One 6:e18365. (Neut)
Product Citations: Liang X, et al. 2022. Cancer Commun (Lond). 42:205. PubMed Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed Russler-Germain EV, et al. 2021. Elife. 10:. PubMed De Ponte Conti B, et al. 2021. Elife. 10:. PubMed Bachy S, et al. 2022. iScience. 25:103758. PubMed Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed Zheng G, et al. 2017. Respir Res. 10.1186/s12931-017-0599-5. PubMed Prakash MD, et al. 2021. PLoS One. 16:e0247492. PubMed Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed Culina S, et al. 2015. Diabetes. 64: 3532 - 3542. PubMed L M, et al. 2016. Brain. 139: 1939-1957. PubMed Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
RRID: AB_2561580 (BioLegend Cat. No. 141407) AB_2650898 (BioLegend Cat. No. 141408)
Structure: Dimmers of latency-associated peptide non-covalently associated with dimmers of mature TGF-β
Distribution: Many cell types, highly expressed on activated Tregs and platelets
Function: TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response
Ligand/Receptor: TGF-β receptors
Cell Type: Dendritic cells, Platelets, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Oida T, et al. 2010. PLoS One 5:e15523. 2. Tran D, et al. 2009. P. Natl. Acad. Sci. USA 106:13445. 3. Ochi H, et al. 2006. Nat. Med. 12:627. 4. Oida T, et al. 2003. J. Immunol. 170:2516. 5. Nakamura K. 2001. J. Exp. Med. 194:629. 6. Miyazono K, et al. 1993. Growth Factors 8:11.
Gene ID: 21803
UniProt: View information about LAP on UniProt.org
Clone: TW7-16B4
Regulatory Status: RUO
Other Names: Latency Associated Peptide (LAP), Transforming growth factor-beta 1 (TGF-b1), TGFB1, DPD1, TGF-beta1
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.