Biolegend, 141605, APC anti-mouse H-2K, 25μg

This antibody has been proven to be very useful in tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo .
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: SIINFEKL pulsed RMA-S cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: The 25-D1.16 monoclonal antibody specifically reacts with ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or H-2Kb bound with an irrelevant peptide. Additional reported applications (for relevant formats) include: immunofluorescence microscopy2,3, immunohistochemical staining of frozen tissue sections3 , and inhibition of T cell response to H-2Kb-SIINFEKL in vitro.
Application References(PubMed link indicates BioLegend citation): Mareeva T, et al. 2010. J. Immunol. Methods 353:78. Dolan BP, et al. 2010. J. Immunol. 184:1419. (IF) Porgador A, et al. 1997. Immunity 6:715. (IF, IHC) Herve J, et al. 2013. J. Immunol. 190:3163. PubMed.
Product Citations: Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed Malka Y, et al. 2022. Mol Cell. 82:3840. PubMed Li J, et al. 2022. Elife. 11:. PubMed Ou W, et al. 2023. Nat Commun. 14:392. PubMed Li Y, et al. 2023. Adv Mater. 35:e2208923. PubMed Hao H, et al. 2023. Nat Biomed Eng. . PubMed Drijvers JM, et al. 2021. Cancer Immunol Res. 9:184. PubMed Champagne J, et al. 2021. Mol Cell. 81:4709. PubMed Yan J, et al. 2022. J Clin Invest. 132:. PubMed Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed Zhang L, et al. 2021. J Immunother Cancer. 9:. PubMed Ylösmäki E, et al. 2021. J Immunother Cancer. 9:. PubMed Pitsch J, et al. 2021. Ann Neurol. 89:666. PubMed Pérez-Girón J, et al. 2015. J Vis Exp. 100: 52803. PubMed Bartok O, et al. 2020. Nature. . PubMed Fusciello M, et al. 2022. Mol Ther Oncolytics. 25:137. PubMed Shen M, et al. 2022. Nat Cancer. 3:60. PubMed Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed Cordeiro B, et al. 2020. Cell Reports. 31(5):107585. PubMed Song TY, et al. 2021. Nat Commun. 12:7003. PubMed Ahn J et al. 2018. Cancer cell. 33(5):862-873 . PubMed Zhang Y, et al. 2022. Nat Commun. 13:4948. PubMed Reguzova A, et al. 2020. Vaccines (Basel). 8:. PubMed Gejman RS et al. 2018. eLife. 7 pii: e41090. PubMed
RRID: AB_11219402 (BioLegend Cat. No. 141605) AB_11219595 (BioLegend Cat. No. 141606)
Structure: OVA peptide257-264 and H-2Kb complex
Distribution: SIINFEKL bond H-2Kb bearing cells
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78. 2. Mareeva T, et al. 2008. J. Biol. Chem. 283:29053. 3. Deng Y, et al. 1998. J. Immunol. 161:1677.
Gene ID: 14972
UniProt: View information about H-2Kb bound to SIINFEKL on UniProt.org
Clone: 25-D1.16
Regulatory Status: RUO
Other Names: MHC class I molecule Kb bound to the peptide SIINFEKL (Kb-SIINFEKL) H-2Kb, OVA agonist peptide (OVAp), OVA-Kb, OVA257-264 (SIINFEKL)-H-2kb, OVA257-264-H-2kb
Isotype: Mouse IgG1, κ