Biolegend, 141721, Brilliant Violet 605™ anti-mouse CD206 (MMR) Antibody, 125microl

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.
125microl
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD206 (MMR)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality testedFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.
Application References(PubMed link indicates BioLegend citation): Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed Yang X, et al. 2015. PNAS. 112:2900. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Zhao Y, et al. 2022. J Neurosci. . PubMed Wang X, et al. 2023. Stem Cell Rev Rep. . PubMed Weitz JR, et al. 2018. Diabetologia. 61:182. PubMed Eddy W, et al. 2017. The Journal of Immunology. 10.4049/jimmunol.1601777. PubMed Jing Li et al. 2018. Immunity. 48(4):773-786 . PubMed Nie M, et al. 2016. Cell Death Dis. 7:e2261. PubMed Stone AEL, et al. 2019. Nat Commun. 10:3649. PubMed Sun L, et al. 2020. J Immunol. 2177:204. PubMed Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed Simões FC, et al. 2020. Nat Commun. 0.875. PubMed Ganguly S, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:891. PubMed Weitz JR, et al. 2020. Diabetes. 69:1206. PubMed Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed Fan ZP, et al. 2021. Front Immunol. 12:743354. PubMed Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
RRID: AB_2562340 (BioLegend Cat. No. 141721)
Structure: Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution: Macrophages, dendritic cells, Langerhans cells, liver endothelial cells
Function: Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor: Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type: Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: CD Molecules
Antigen References: 1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501. 2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469. 3. Burgdorf S, et al. 2006. J. Immunol. 176:6770. 4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.
Gene ID: 17533
UniProt: View information about CD206 on UniProt.org
Clone: C068C2
Regulatory Status: RUO
Other Names: MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype: Rat IgG2a, κ
Q: Why is mouse CD206 stained intracellularly and not via surface staining?
A: Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.