Biolegend, 141805, PE anti-mouse Allergin-1 Antibody, 25μg

Allergin-1, also known as mast cell Ag-32, is a 60 kD single pass transmembrane protein. It contains one Ig-like C2-type (immunoglobulin-like) domain in the extracellular region and one immunoreceptor tyrosine-based inhibitory motif (ITIM)-like domain in the cytoplasmic region. Allergin-1 is expressed on mast cells, dendritic cells, and macrophages as well as at low levels on neutrophils. After tyrosine phosphorylation, allergin-1 recruits PTPN6, PTPN11, and INPP5D, which results in a diminution of IgE-mediated degranulation in mast cells. No ligands have been identified for this molecule.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Transfected cells expressing allergin-1-Flag
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Inhibits FcεRI-mediated degranulation in bone marrow-derived cultured mast cells1. Additional reported applications (for the relevant formats) include: blocking1.
Application References(PubMed link indicates BioLegend citation): Hitomi K, et al. 2010. Nat. Immunol. 11:601. (Block)
RRID: AB_10900438 (BioLegend Cat. No. 141805)
Structure: Single pass transmembrane protein of 60 kD; contains one Ig-like C2-like domain and one ITIM-like domain
Distribution: Mast cells, dendritic cells, macrophages, neutrophils
Function: Inhibits IgE-mediated degranulation of mast cells
Interaction: PTPN6, PTPN11 and INPP5D
Cell Type: Dendritic cells, Macrophages, Mast cells, Neutrophils
Biology Area: Cell Biology, Immunology, Signal Transduction
Antigen References: 1. Hitomi K, et al. 2010. Nat. Immunol. 11:601. 2. Pirozzi G, et al. 1995. J. Immunol. 155:5811.
Gene ID: 380732
UniProt: View information about Allergin-1 on UniProt.org
Clone: TX83
Regulatory Status: RUO
Other Names: Allergy inhibitory receptor 1, mast cell Ag-32, MCA-32, Milr1
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.