Biolegend, 141809, Brilliant Violet 421™ anti-mouse Allergin-1 Antibody, 50μg

Allergin-1, also known as mast cell Ag-32, is a 60 kD single pass transmembrane protein. It contains one Ig-like C2-type (immunoglobulin-like) domain in the extracellular region and one immunoreceptor tyrosine-based inhibitory motif (ITIM)-like domain in the cytoplasmic region. Allergin-1 is expressed on mast cells, dendritic cells, and macrophages as well as at low levels on neutrophils. After tyrosine phosphorylation, allergin-1 recruits PTPN6, PTPN11, and INPP5D, which results in a diminution of IgE-mediated degranulation in mast cells. No ligands have been identified for this molecule.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Transfected cells expressing allergin-1-Flag
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Inhibits FcεRI-mediated degranulation in bone marrow-derived cultured mast cells1. Additional reported applications (for the relevant formats) include: blocking1.
Application References(PubMed link indicates BioLegend citation): Hitomi K, et al. 2010. Nat. Immunol. 11:601. (Block)
RRID: AB_3674992 (BioLegend Cat. No. 141809)
Structure: Single pass transmembrane protein of 60 kD; contains one Ig-like C2-like domain and one ITIM-like domain
Distribution: Mast cells, dendritic cells, macrophages, neutrophils
Function: Inhibits IgE-mediated degranulation of mast cells
Interaction: PTPN6, PTPN11 and INPP5D
Cell Type: Dendritic cells, Macrophages, Mast cells, Neutrophils
Biology Area: Cell Biology, Immunology, Signal Transduction
Antigen References: 1. Hitomi K, et al. 2010. Nat. Immunol. 11:601. 2. Pirozzi G, et al. 1995. J. Immunol. 155:5811.
Gene ID: 380732
UniProt: View information about Allergin-1 on UniProt.org
Clone: TX83
Regulatory Status: RUO
Other Names: Allergy inhibitory receptor 1, mast cell Ag-32, MCA-32, Milr1
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.