Biolegend, 141906, APC anti-mouse CD283 (TLR3) Antibody, 100μg

Toll-like receptor 3 (TLR3), also known as CD283, is type I transmembrane protein that belongs to the TLR family. It forms a large horseshoe shape that contacts a neighboring horseshoe, forming a dimer of two horseshoes. It is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. TLR3 is expressed selectively on the cytoplasmic membrane and intracellularly in dendritic cells. It is also highly expressed in the placenta, pancreas, heart, liver, lung, and muscle. TLR3 is a pattern recognition receptor that participates in innate immune response to microbial pathogens by recognizing polyinosine-polycytidylic acid (Poly(I:C)) and dsRNA. Ligand binding by TLR3 induces receptor dimerization which results in inducing NF-κB activation (via TRIF-linked RIP1/TLR3 interactions) and cytokine production. TLR3 has been shown to interact with a number of proteins including MYD88, TRAF6, TRIAD3, MAP3K7, and TAB2.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse TLR3 extracellular domain.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue1.
Application References(PubMed link indicates BioLegend citation): McCartney S, et al. 2009. J. Exp. Med. 13:2967. (IHC)
Product Citations: Zhou L, et al. 2022. PLoS Negl Trop Dis. 16:e0010851. PubMed Silver AC, et al. 2018. Heliyon. 4:e00579. PubMed Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed Takemura R, et al. 2015. Cancer Immunol Res. 3: 902-914. PubMed Berrien–Elliott MM, et al. 2020. Immunity. 51(3):479-490. PubMed
RRID: AB_2561436 (BioLegend Cat. No. 141905) AB_2561437 (BioLegend Cat. No. 141906)
Structure: Toll-like receptor family member; type I membrane protein containing multiple leucine repeats and a interleukin-1 receptor like region in the extracellular domain. Predicted molecular weight approximately 116 kD.
Distribution: Selective expression in dendritic cells. Highly expressed in placenta and the pancreas; also expressed in heart, liver, lung and muscle.
Function: Pattern recognition receptor that participates in innate immune response to microbial pathogens. TLR3 ligands induce NF-κB (via TRIF-linked RIP1/TLR3 interactions) and cytokine production. TLR3 stimulation results in the production of cytokines.
Interaction: MYD88, TRAF6, TRIAD3, MAP3K7, and TAB2.
Ligand/Receptor: Polyinosine-polycytidylic acid (Poly(I:C)), dsRNA.
Cell Type: Dendritic cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: CD Molecules, Toll Like Receptors
Antigen References: 1. Alexopoulou L, et al. 2001. Nature 413:732.2. Doyle SE, et al. 2002. Immunity 17:251.3. Meylan E, et al. 2004. Nat Immunol. 5:503.4. Muzio M, et al. 2000. J. Immunol. 164:5998.5. Rock FL, et al. 1998. P. Natl. Acad. Sci. USA 95:588.
Gene ID: 142980
UniProt: View information about CD283 on UniProt.org
Clone: 11F8
Regulatory Status: RUO
Other Names: Toll like receptor 3
Isotype: Rat IgG2a