Biolegend, 142002, Purified anti-mouse CD355 (CRTAM) Antibody, 100μg

Class-I MHC-restricted T-cell associated molecule (CRTAM), designated as CD355, is an Ig domain like type I transmembrane receptor, which belongs to the nectin family. In the immune system, CRTAM is strictly expressed on activated NK cells and activated CD8-positive T cells. By binding its ligand Nectin-like molecule 2 (Necl2), CRTAM plays an important role in mediating cell adhesion and migration.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CRTAM extracellular region-human IgG Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedWB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for relevant formats) include: Western blotting1.
Application References(PubMed link indicates BioLegend citation): Arase N, et al. 2005. Int. Immunol. 17:1227. (WB)
RRID: AB_10899412 (BioLegend Cat. No. 142002)
Structure: 75 kD homodimer, nectin family, Ig superfamily
Distribution: Activated NK cells and activated CD8-positive T cells
Function: Cell adhesion, migration
Ligand/Receptor: Nectin-like molecule 2
Cell Type: NK cells, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Takeuchi A, et al. 2009. J. Immunol. 183:4220. 2. Yeh JH, et al. 2008. Cell 132:846. 3. Arase N. 2005. Int. Immunol. 17:1227. 4. Boles KS, et al. 2005. Blood 106:779. 5. Galibert L, et al. 2005 J. Biol. Chem. 280:21955. 6. Kennedy J, et al. 2000 J. Leukoc. Biol. 67:725.
Gene ID: 54698
UniProt: View information about CD355 on UniProt.org
Clone: 11-5/CRTAM
Regulatory Status: RUO
Other Names: Class I-restricted T cell-associated molecule
Isotype: Rat IgG2a, κ