Biolegend, 142301, Purified anti-mouse CD200R3 Antibody, 50μg

CD200R3, also known as CD200RLb and OX-2 Receptor 3, is a disulfide-linked dimmeric CD200R-like receptor which belongs to immunoglobulin superfamily.  Its positively charged aminio acid lysinein associates with ITAM- or YxxM motif-bearing adaptor molecules such as DAP12, DAP10, FcRγ, and CD3ζ. CD200R3 functions as an activating receptor to regulate IgE independent immune response.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse primary basophils
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested Depletion - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Ba160 recognizes circulating and bone marrow basophils; it also recognizes a subset of mast cells in the peritoneal cavity and skin. A single intravenous administration of 30 µg of LEAF Ba160 to mice induces a dramatic reduction of basophil number in peripheral blood from day 1 to day 10. *Tested in the development stage.
RRID: AB_10915471 (BioLegend Cat. No. 142302)
Structure: Immunoglobulin superfamily; disulfide-linked dimmer with positively charged amino acids in transmembrane; molecular weight is about ~38 kD or ~74 kD (reduced or non-reduced condition, respectively).
Distribution: Basophils in peripheral blood, bone marrow, spleen: mast cells in peritoneal cavity and skin.
Function: Transduce stimulatory signals.
Interaction: DAP12, DAP10, FcRγ, or CD3ζ
Cell Type: Basophils, Mast cells
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Voehringer D, et al. 2004. J. Biol. Chem. 52:54117. 2. Kojima T, et al. 2007. J. Immunol. 179:7093. 3. Sato K, et al. 2009. Blood 113:4780.
Gene ID: 74603
UniProt: View information about CD200R3 on UniProt.org
Clone: Ba160
Regulatory Status: RUO
Other Names: CD200 R3, CD200RLb, OX-2 Receptor 3, CD200 receptor 3
Isotype: Rat IgG2b, κ