Biolegend, 142411, PE/Cyanine7 anti-mouse CD169 (Siglec-1) Antibody, 25μg

CD169, also known as Siglec-1 and Sialoadhesin (Sn), is a type I lectin containing 17 immunoglobulin (Ig) domains (one variable domain and 16 constant domains). CD169 binds to sialic acids, which can be found on PSGL-1, CD43, CD206, and CD227. By its affinity to α2, 3-linked sialic acid, it is involved in macrophage binding to different cell types such as granulocytes, monocytes, NK, B, and T cells. CD169 was initially identified as a sialic acid-dependent sheep erythrocyte receptor (SER) on resident bone marrow cells of mice. It has been identified as highly expressed on resident bone marrow macrophages which plays an important role in retention of stem cells in mesenchymal stem cell niche. It is also found on some specific subsets of tissue macrophages in spleen, lymph nodes, bone marrow, liver, colon, lungs, and cancer cells. Evidence suggest that CD169-positive macrophages serve as lymph node-resident APCs to dominate early activation of tumor antigen-specific CD8 + T cells and invariant NK cell.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Purified Native Sialoadhesin from spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining in frozen tissue sections1, and spatial biology (IBEX)4,5.
Application References(PubMed link indicates BioLegend citation): Barral P, et al. 2010. Nat. Immunol. 11:303. (IHC-F) Chtanova T, et al. 2008. Immunity 29:487. Klass M, et al. 2012. J. Immunol. 189:2414. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Backlund C, et al. 2022. Proc Natl Acad Sci U S A. 119:e2204078119. PubMed Huseni MA, et al. 2023. Cell Rep Med. 4:100878. PubMed Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed Grigoryan L, et al. 2022. NPJ Vaccines. 7:55. PubMed Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed Nahrendorf W, et al. 2021. eLife. 10:00. PubMed Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed Hsia HC, et al. 2017. J Leukoc Biol. 101:1053. PubMed Smith LK, et al. 2021. Elife. 10:. PubMed Lee A, et al. 2022. Nat Commun. 13:549. PubMed Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
RRID: AB_2563910 (BioLegend Cat. No. 142411) AB_2563911 (BioLegend Cat. No. 142412)
Structure: Type I single membrane-spanning lectin containing 17 immunoglobulin (Ig) domains, belongs to the immunoglobulin superfamily.
Distribution: Macrophages in spleen, lymph nodes, bone marrow, liver, colon and lungs.
Function: Adhesion.
Ligand/Receptor: PSGL-1, CD43, CD206 and CD227.
Cell Type: Macrophages
Biology Area: Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase, Siglec Molecules
Antigen References: 1. Chow A, et al. 2011. J. Exp. Med. 208:261. 2. Asano K, et al. 2011. Immunity 34:85. 3. Xiong YS, et al. 2009. Clin. Biochem. 42:1057. 4. Varki A, et al. 2009. Glycoconj. J. 26:231. 5. Rempel H, et al. 2008. PLoS One 3:e1967. 6. Crocker PR, et al. 2001. Trends Immunol. 22:337. 7. Hartnell A, et al. 2001. Blood 97:288. 8. Crocker PR, et al. 1985. J. Exp. Med. 162:993.
Gene ID: 20612
UniProt: View information about CD169 on UniProt.org
Clone: 3D6.112
Regulatory Status: RUO
Other Names: Sialic acid binding Ig-like lectin 1 (Siglec-1), Sialoadhesin (Sn)
Isotype: Rat IgG2a, κ