Biolegend, 142416, Alexa Fluor® 594 anti-mouse CD169 (Siglec-1) Antibody, 100μg

CD169, also known as Siglec-1 and Sialoadhesin (Sn), is a type I lectin containing 17 immunoglobulin (Ig) domains (one variable domain and 16 constant domains). CD169 binds to sialic acids, which can be found on PSGL-1, CD43, CD206, and CD227. By its affinity to α2, 3-linked sialic acid, it is involved in macrophage binding to different cell types such as granulocytes, monocytes, NK, B, and T cells. CD169 was initially identified as a sialic acid-dependent sheep erythrocyte receptor (SER) on resident bone marrow cells of mice. It has been identified as highly expressed on resident bone marrow macrophages which plays an important role in retention of stem cells in mesenchymal stem cell niche. It is also found on some specific subsets of tissue macrophages in spleen, lymph nodes, bone marrow, liver, colon, lungs, and cancer cells. Evidence suggest that CD169-positive macrophages serve as lymph node-resident APCs to dominate early activation of tumor antigen-specific CD8 + T cells and invariant NK cell.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Purified Native Sialoadhesin from spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-F - Quality tested 3D IHC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 µg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining in frozen tissue sections1, and spatial biology (IBEX)4,5.
Application References(PubMed link indicates BioLegend citation): Barral P, et al. 2010. Nat. Immunol. 11:303. (IHC-F) Chtanova T, et al. 2008. Immunity 29:487. Klass M, et al. 2012. J. Immunol. 189:2414. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Zhang M, et al. 2023. Int J Biol Sci. 19:1698. PubMed Martens R, et al. 2020. Nat Commun. 11:1114. PubMed Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed Enders M, et al. 2020. J Immunol. 204:87. PubMed
RRID: AB_2565620 (BioLegend Cat. No. 142416)
Structure: Type I single membrane-spanning lectin containing 17 immunoglobulin (Ig) domains, belongs to the immunoglobulin superfamily.
Distribution: Macrophages in spleen, lymph nodes, bone marrow, liver, colon and lungs.
Function: Adhesion.
Ligand/Receptor: PSGL-1, CD43, CD206 and CD227.
Cell Type: Macrophages
Biology Area: Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase, Siglec Molecules
Antigen References: 1. Chow A, et al. 2011. J. Exp. Med. 208:261. 2. Asano K, et al. 2011. Immunity 34:85. 3. Xiong YS, et al. 2009. Clin. Biochem. 42:1057. 4. Varki A, et al. 2009. Glycoconj. J. 26:231. 5. Rempel H, et al. 2008. PLoS One 3:e1967. 6. Crocker PR, et al. 2001. Trends Immunol. 22:337. 7. Hartnell A, et al. 2001. Blood 97:288. 8. Crocker PR, et al. 1985. J. Exp. Med. 162:993.
Gene ID: 20612
UniProt: View information about CD169 on UniProt.org
Clone: 3D6.112
Regulatory Status: RUO
Other Names: Sialic acid binding Ig-like lectin 1 (Siglec-1), Sialoadhesin (Sn)
Isotype: Rat IgG2a, κ