Biolegend, 142502, Purified anti-mouse CD138 (Syndecan-1) Antibody, 500μg

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse mammary gland epithelial cell line NMuMG
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICC, IHC-P, IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.
Application References(PubMed link indicates BioLegend citation): Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC) Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF) Li Q, et al. 2002. Cell 111:635. (IF, IHC) McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
Product Citations: Papoutsopoulou S, et al. 2022. Am J Physiol Gastrointest Liver Physiol. 323:G306. PubMed Kobayashi A, et al. 2021. Front Immunol. 12:650856. PubMed Valet C, et al. 2022. J Clin Invest. 132: . PubMed Agrafiotis A, et al. 2023. Acta Neuropathol. 145:335. PubMed Yao W, et al. 2019. Nature. 568:410. PubMed Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed McBride KM, et al. 2019. Front Oncol. 9:517. PubMed Tran JQ, et al. 2020. Blood Adv. 4:5547. PubMed Akhmetzyanova I, et al. 2020. Leukemia. 34:245. PubMed Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed Majer O, et al. 2019. Nature. 575:366. PubMed Park PW, et al. 2018. Methods Cell Biol. 143:317. PubMed Cui X, et al. 2017. J Immunol. 199:4066. PubMed Platteel ACM, et al. 2018. Front Immunol. 1.544444444. PubMed Prabakaran T, et al. 2021. EBioMedicine. 66:103314. PubMed
RRID: AB_10959655 (BioLegend Cat. No. 142501) AB_10965646 (BioLegend Cat. No. 142502)
Structure: Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function: Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type: B cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Zong F, et al. 2011. PLoS ONE 6:e14816. 2. Yamashita Y, et al. 1999. J. Immunol. 162:5940. 3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.
Gene ID: 20969
UniProt: View information about CD138 on UniProt.org
Clone: 281-2
Regulatory Status: RUO
Other Names: Syndecan-1
Isotype: Rat IgG2a, κ