Biolegend, 142506, APC anti-mouse CD138 (Syndecan-1) Antibody, 100μg

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse mammary gland epithelial cell line NMuMG
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.
Application References(PubMed link indicates BioLegend citation): Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC) Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF) Li Q, et al. 2002. Cell 111:635. (IF, IHC) McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
Product Citations: Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed Wang YC, et al. 2023. J Exp Med. 220:. PubMed Mi Z, et al. 2021. Vaccines (Basel). 10: . PubMed Su C, et al. 2022. NPJ Vaccines. 7:168. PubMed Kumpunya S, et al. 2023. Front Immunol. 13:1010764. PubMed Xiong E, et al. 2023. Front Immunol. 13:1083119. PubMed Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed Yao W, et al. 2019. Nature. 568:410. PubMed Delgado-Benito V, et al. 2020. J Exp Med. 217:00:00. PubMed Parthasarathy R, et al. 2021. Front Immunol. 12:758407. PubMed Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed Xiong Y, et al. 2018. Int J Clin Exp Pathol. 3.803472222. PubMed Wang Z, et al. 2021. J Virol. 95:e0141421. PubMed Utley A, et al. 2020. Cell Rep. 31:107815. PubMed Ray A, et al. 2015. PLoS One. 10: 0137314. PubMed Qi T, et al. 2020. Cell Rep. 33:108452. PubMed Chen F et al. 2018. Cell reports. 25(10):2775-2783 . PubMed Li Z, et al. 2021. Cell Metabolism. 33(1):78-93.e7. PubMed Zhang YN, et al. 2020. EBioMedicine. 56:102819. PubMed Roy K, et al. 2019. Immunity. 50:616. PubMed Chiu Y, et al. 2014. J Immunol. 193:2207. PubMed Minamitani T, et al. 2015. Proc Natl Acad Sci U S A. 112: 11612-11617. PubMed Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed Castiblanco D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01833. PubMed Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed Chen C, et al. 2021. J Virol. 95:e0082921. PubMed Zavidij O, et al. 2020. Nat Cancer. 0.384027778. PubMed Han S, et al. 2016. Arthritis Res Ther. 0.975. PubMed Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed Cillo AR, et al. 2021. Cell Rep Med. 2:100476. PubMed Price MJ, et al. 2019. J Immunol. 203:2121. PubMed Vijay R, et al. 2021. J Exp Med. 218:. PubMed Wright JA, et al. 2021. Front Immunol. 12:705307. PubMed Xiong Y, et al. 2019. BMC Immunol. 20:24. PubMed Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed Zhang Y, et al. 2022. Front Pharmacol. 13:952775. PubMed Lightman SM, et al. 2020. Cell Reports. 1.086805556. PubMed Zhang M, et al. 2022. iScience. 25:104490. PubMed Alrefai H, et al. 2016. Nat Commun. 7:11724. PubMed Whiteley AM et al. 2017. eLife. 6 pii: e26435. PubMed
RRID: AB_10960141 (BioLegend Cat. No. 142505) AB_10962911 (BioLegend Cat. No. 142506)
Structure: Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function: Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type: B cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Zong F, et al. 2011. PLoS ONE 6:e14816. 2. Yamashita Y, et al. 1999. J. Immunol. 162:5940. 3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.
Gene ID: 20969
UniProt: View information about CD138 on UniProt.org
Clone: 281-2
Regulatory Status: RUO
Other Names: Syndecan-1
Isotype: Rat IgG2a, κ