Biolegend, 142518, Brilliant Violet 650™ anti-mouse CD138 (Syndecan-1) Antibody, 50μg

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse mammary gland epithelial cell line NMuMG
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.4 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.
Application References(PubMed link indicates BioLegend citation): Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC) Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF) Li Q, et al. 2002. Cell 111:635. (IF, IHC) McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
Product Citations: Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed Schiepers A, et al. 2023. Nature. 615:482. PubMed Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
RRID: AB_2562404 (BioLegend Cat. No. 142517) AB_2650927 (BioLegend Cat. No. 142518)
Structure: Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function: Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type: B cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Zong F, et al. 2011. PLoS ONE 6:e14816. 2. Yamashita Y, et al. 1999. J. Immunol. 162:5940. 3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.
Gene ID: 20969
UniProt: View information about CD138 on UniProt.org
Clone: 281-2
Regulatory Status: RUO
Other Names: Syndecan-1
Isotype: Rat IgG2a, κ