Biolegend, 142530, APC/Cyanine7 anti-mouse CD138 (Syndecan-1) Antibody, 100μg

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse mammary gland epithelial cell line NMuMG
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.
Application References(PubMed link indicates BioLegend citation): Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC) Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF) Li Q, et al. 2002. Cell 111:635. (IF, IHC) McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
Product Citations: Mathur S, et al. 2021. JCI Insight. 6:. PubMed Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed Dotsey E, et al. 2017. Sci Rep. 7:42584. PubMed Alenazy MF, et al. 2022. PLoS One. 17:e0271689. PubMed Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
RRID: AB_2566581 (BioLegend Cat. No. 142529) AB_2566582 (BioLegend Cat. No. 142530)
Structure: Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function: Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type: B cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Zong F, et al. 2011. PLoS ONE 6:e14816. 2. Yamashita Y, et al. 1999. J. Immunol. 162:5940. 3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.
Gene ID: 20969
UniProt: View information about CD138 on UniProt.org
Clone: 281-2
Regulatory Status: RUO
Other Names: Syndecan-1
Isotype: Rat IgG2a, κ