Biolegend, 142703, PE anti-mouse TCR Vγ1.1 + Vγ1.2 Antibody, 25μg

In mice, the TCR γ locus consists of seven Vγ, four Jγ, and four Cγ genes, which are differentially expressed throughout their lifespan. During fetal and early postnatal life, very few γ/δ T cells express TCR Vγ1.1 or Vγ1.2 chains. In adult mice, however, a major fraction of the γ/δ T cell repertoire comprises either TCR Vγ1.1 or Vγ1.2 chains. The Vγ1.1 gene rearranges almost exclusively with the Jγ4-Cγ4 genes, while the Vγ1.2 gene preferentially rearranges with Jγ1. The 4B2.9 antibody reacts specifically with mouse TCR Vγ1.1 and Vγ1.2 as designated by the Garman nomenclature system. Mouse TCR Vγ1.1 and Vγ1.2 are also known as TCR Vγ1 and Vγ2, respectively, under the Heilig & Tonegawa nomenclature system. Several TCR γ and δ chain nomenclature systems exist. In order to consolidate the various nomenclature systems, we have created charts for corresponding names across some of the most popular naming methods. Click here to see charts .
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: TCR Vγ1.1/Vδ6 T3.13.1 hybridoma
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: mAb 4B2.9 specifically recognizes γ/δ T cells expressing Vγ1.1 and/or Vγ1.2 chains.
Application References(PubMed link indicates BioLegend citation): Pereira P and Boucontet L. 2004. J. Immunol. 173:3261. (FC)
Product Citations: Giampaolo S, et al. 2023. iScience. 26:106234. PubMed Bachy E, et al. 2016. J Exp Med. 213: 841 - 857. PubMed
RRID: AB_10960739 (BioLegend Cat. No. 142703) AB_10962688 (BioLegend Cat. No. 142704)
Distribution: Subset of γ/δ T cells
Function: Antigen recognition
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Garcia-Herrera A, et al. 2011. Am. J. Surg. Pathol. 35:1214. 2. Gutierrez A, et al. 2010. J. Clin. Oncol. 28:3816. 3. Zhang X, et al. 2009. Hematology 14:237.
Gene ID: 110067
UniProt: View information about TCR Vgamma1.1 1.2 on UniProt.org
Clone: 4B2.9
Regulatory Status: RUO
Other Names: Vγ1 and Vγ2 (Tonegawa nomenclature), TRGV1, TRGV2, GV5S1, GV5S2
Isotype: Armenian Hamster IgG
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.