Biolegend, 142903, PE anti-mouse GARP (LRRC32) Antibody, 25μg

Glycoprotein A Repetitions Predominant (GARP), also known as leucine rich repeat containing protein 32 (LRC32), is a 80 kD type I membrane glycoprotein with 20 leucine rich repeats in the extracellular portion of the protein. GARP was found on the surface of megakaryocytes, platelets, and activated Tregs. It serves as a receptor for latent TGF-β. Recent evidence suggests that GARP may play a role in controlling suppressor function of Tregs. A mutation in GARP has been reported in a large Samaritan kindred with Usher syndrome type 1. In addition, it has been found that GARP mRNA is highly amplified in different tumors, which indicates that tumor cells may use GARP to express TGF-β or to capture TGF-β from their surroundings, resulting in local suppression of anti-tumor immune responses or the induction of Tregs.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse GARP (extracellular domain)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed Geng S, et al. 2019. Sci Adv. 5:eaav2309. PubMed Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
RRID: AB_10983065 (BioLegend Cat. No. 142903) AB_10962944 (BioLegend Cat. No. 142904)
Structure: 80 kD transmembrane GARP glycoprotein with an extracellular region containing 20 leucine-rich repeats
Distribution: Activate Tregs, megakaryocytes, platelets
Ligand/Receptor: LAP (TGF-β1)
Cell Type: Tregs, Megakaryocytes, Platelets
Biology Area: Cell Biology, Immunology, Signal Transduction
Antigen References: 1. Ollendorff V, et al. 1994. Cell. Growth Differ. 5:213. 2. Stockis J, et al. 2009. Eur. J. Immunol. 39:3315. 3. Wang R, et al. 2009. P. Natl. Acad. Sci. USA 106:13439. 4. Tran DQ, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.
Gene ID: 434215
UniProt: View information about GARP on UniProt.org
Clone: F011-5
Regulatory Status: RUO
Other Names: LRRC32, Garpin, Glycoprotein A repetitions predominant
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.