Biolegend, 143007, PerCP/Cyanine5.5 anti-mouse CD160 Antibody, 25μg

CD160, also known as BY55, is a 27 kD glycoprotein and member of the Ig superfamily. It is anchored to the cell membrane through glycosylphosphatidylinositol (GPI) and forms disulfide-linked multimers. A soluble form of CD160 is secreted by activated CD8 + T cells. Expressed by NK, NKT, γ/δ T cells, intestinal intraepithelial T cells and a subset of memory CD8 + T cells, CD160 binds both classical and non-classical MHC class I molecules. It is also a ligand for HVEM. CD160 enhances proliferation of activated CD8 + T cells and triggers cell cytotoxicity in NK cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Soluble His-Tag mouse CD160
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Tsujimura K, et al. 2006. Immunol. Lett. 106:48. (FC)
Product Citations: Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed Pauken KE, et al. 2020. Cell Reports. 31(13):107827. PubMed Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
RRID: AB_2562675 (BioLegend Cat. No. 143007) AB_2562676 (BioLegend Cat. No. 143008)
Structure: Membrane glycoprotein, member of the Ig superfamily, 27 kD, glycosylphosphatidylinositol (GPI)-anchored, forms disulfide-linked multimers on the cell surface
Distribution: NK, NKT, γ/δT cells, intestinal intraepithelial T cells, subset of memory CD8+ T cells
Function: Enhances proliferation of activated CD8+ T cells, triggers cell cytotoxicity in NK cells
Ligand/Receptor: Classical and non-classical MHC-class I molecules, HVEM
Cell Type: NK cells, NKT cells, T cells
Biology Area: Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: CD Molecules, MHC Antigens, TCRs
Antigen References: 1. Shui JW, et al. 2011. J. Leukoc. Biol. 89:517. 2. Del Rio ML, et al. 2010. J. Leukoc. Biol. 87:223. 3. Cai G and Freeman GJ. 2009. Immunol. Rev. 229:244.
Gene ID: 54215
UniProt: View information about CD160 on UniProt.org
Clone: 7H1
Regulatory Status: RUO
Other Names: BY55, NK1, NK28
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.