Biolegend, 143303, PE anti-mouse Trem-like 4 (Treml4) Antibody, 25μg

Trem-like 4, a type I transmembrane protein and a member of the TREM family, consists of a single extracellular Ig-like domain of the V-type, a transmembrane domain, and a short cytoplasmic domain. Trem-like 4 is expressed on macrophages in the spleen, and dendritic cells, especially CD8+ dendritic cells. It is proposed to regulate the immune response based on its expression profile. It has been reported that B7-H3 is a receptor and that the soluble form of Treml4 has a binding affinity to late apoptotic or necrotic cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Extracellular domain of recombinant mouse Trem-like 4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescent staining, immunoprecipitation, and Western blotting1.
Application References(PubMed link indicates BioLegend citation): Hemmi H, et al. 2009. J. Immunol. 182:1278. (WB)
Product Citations: Slusarczyk P, et al. 2023. Elife. 12: . PubMed Devalaraja S, et al. 2020. Cell. 1098:180. PubMed Pek RH, et al. 2019. Elife. 8:e49503. PubMed Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
RRID: AB_11126143 (BioLegend Cat. No. 143303) AB_11125372 (BioLegend Cat. No. 143304)
Structure: Type I transmembrane protein; member of the TREM family
Distribution: CD8+ dendritic cells, dendritic cell subsets, macrophages in the spleen, myeloid cells
Function: Regulates innate immune response and homeostatis
Interaction: Associates with DAP12; binds late apoptotic cells/necrotic cells
Cell Type: B cells, Dendritic cells, Macrophages
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Ford JW and McVicar DW. 2009. Curr. Opin. Immunol. 21:38. 2. Hemmi H, et al. 2009. J. Immunol. 182:1278.
Gene ID: 224840
UniProt: View information about Trem-like 4 on UniProt.org
Clone: 16E5
Regulatory Status: RUO
Other Names: Trem-l4, TREM like transcript 4, triggering receptor expressed on myeloid cells-like 4
Isotype: Rat IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.