Biolegend, 143502, Purified anti-mouse CD370 (CLEC9A, DNGR1) Antibody, 100μg

CD370 (CLEC9A), also known as DNGR1, is a 264 amino acid (aa) type II transmembrane glycoprotein which belongs to the C-type lectin superfamily. It contains a 208 aa extracellular region with a single carbohydrate recognition domain (CRD) and a cytoplasmic segment (35 aa) bearing an immunoreceptor tyrosine-based activation motif (ITAM).  In mice, CLEC9A is strictly expressed on CD8a-positive dendritic cells and plasmacytoid dendritic cells. It recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedELISA, ICC, IP, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
Application Notes: Additional reported applications (for the relevant formats) include: ELISA2, immunofluorescence microcopy1, immunoprecipitation1, and Western blotting1.
Application References(PubMed link indicates BioLegend citation): Sancho D, et al. 2008. J. Clin. Invest. 118:2098. (IF, IP, WB) Joffre OP, et al. 2010. Eur. J. Immunol. 40:1255. (ELISA)
Product Citations: Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
RRID: AB_11125169 (BioLegend Cat. No. 143502)
Structure: Type II transmembrane glycoprotein, consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD)
Distribution: Strictly expressed on CD8α+ dendritic cells, migratory CD103+ dendritic cells, and plasmacytoid dendritic cells
Function: Cross present antigen to CTL
Interaction: Necrotic cells
Ligand/Receptor: Ubiquitous preformed acid-labile protein associated ligand(s)
Cell Type: Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, MHC Antigens
Antigen References: Poulin LF, et al. 2012. Blood 119:6052.
Gene ID: 232414
UniProt: View information about CD370 on UniProt.org
Clone: 7H11
Regulatory Status: RUO
Other Names: DNGR-1, C-type lectin domain family 9 member A, CD370
Isotype: Rat IgG1, κ