Biolegend, 144104, PE anti-mouse PIR-A/B Antibody, 100μg

Paired-immunoglobulin-like receptor (PIR) is a type I transmembrane protein, containing six Ig-like domains. Two isoforms (PIR-A and PIR-B) have been reported for this molecule. PIR-A/B is expressed on B cells, mast cells, macrophages, granulocytes, eosinophils, and dendritic cells. PIR-A requires association with FcRγc for its cell surface expression and to act as an activating receptor. The ligand for PIR-A is MHC class I. PIR-B is an inhibitory receptor with 3 ITIM motifs on its cytoplasmic tail; when phosphorylated, they recruit the tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. MHC class I, Nogo, MAG, and OMgp are the ligands for PIR-B.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant extracellular domains 1 and 2 of PIR-A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications for the relevant formats include: Western Blotting1 and immunohistochemical staining of frozen tissue sections3.
Application References(PubMed link indicates BioLegend citation): Kubagawa H, et al. 1999. J. Exp. Med. 189:309. (WB) Nakamura A, et al. 2004. Nat. Immunol. 5:623. (FC) Masuda K, et al. 2005. EMBO J. 24:4052. (IHC)
Product Citations: Ye Y, et al. 2017. Sci Immunol. 2:17. PubMed
RRID: AB_11203371 (BioLegend Cat. No. 144103) AB_11204242 (BioLegend Cat. No. 144104)
Structure: Transmembrane proteins, six Ig-like domains. PIR-A associates with FcRγc, doesn't have functional motifs on its cytoplasmic domain. PIR-B contains 3 ITIM motifs on its cytoplasmic domain.
Distribution: B cells, macrophages, dendritic cells, mast cells, eosinophils.
Interaction: PIR-A: FcRγcPIR-B: Lyn, PTPN6/SHP-1, PTPN11/SHP-2
Ligand/Receptor: MHC class I, Nogo, MAG, OMgp.
Cell Type: B cells, Dendritic cells, Eosinophils, Macrophages, Mast cells, Neutrophils
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: MHC Antigens, Protein Kinases/Phosphatase
Antigen References: 1. Matsushita H, et al. 2011. J. Biol. Chem. 286:25739. 2. Arita K, et al. 2011. J. Immunol. 186:7050. 3. Takai T, et al. 2011. J. Biomed. Biotechnol. 2011:275302. 4. Nakamura A, et al. 2004. Nat. Immunol. 5:623.
Gene ID: 1872218733
UniProt: View information about PIR-A/B on UniProt.org
Clone: 6C1
Regulatory Status: RUO
Other Names: PIRA1, PIR-B Leukocyte immunoglobulin-like receptor 3, LIR-3
Isotype: Rat IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.