Biolegend, 144215, PerCP/Cyanine5.5 anti-mouse/human CD207 (Langerin) Antibody, 25μg

CD207, also known as langerin, is a 40 kD single-pass type II transmembrane protein, member of the C-type lectin family. CD207 is expressed on the cell surface and Birbeck granules (BGs) of Langerhans cells, and subsets of thymic and splenic dendritic cells. Its ligands are mannose, n -acetylglucosamine, fucose, and sulfated glycans. Langerin is involved in the antigen processing pathway through capture and internalization of its ligands.
25μg
Verified Reactivity: Mouse, Human
Reported Reactivity: Cynomolgus, Rabbit, Pig
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Langerin extracellular domain-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_2924476 (BioLegend Cat. No. 144215) AB_2924476 (BioLegend Cat. No. 144216)
Structure: Single-pass type II transmembrane protein, 40 kD, one C-type lectin domain, homotrimer
Distribution: Langerhans cells, subsets of thymic and splenic dendritic cells
Function: Antigen uptake, antigen processing
Ligand/Receptor: Mannose, n-acetylglucosamine, fucose, sulfated glycans
Cell Type: Dendritic cells, Langerhans cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Romani N, et al. 2012. Curr. Top. Microbiol. Immunol. 351:113. 2. Kaplan DH. 2010. Trends Immunol. 31:446. 3. Clausen BE and Kel JM. 2010. Immunol. Cell. Biol. 88:351. 4. Merad M, et al. 2008. Nat. Rev. Immunol. 8:935.
Gene ID: 24627850489
UniProt: View information about CD207 on UniProt.org
Clone: 4C7
Regulatory Status: RUO
Other Names: CLEC4K
Isotype: Mouse IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.