Biolegend, 144509, FITC anti-mouse CD193 (CCR3) Antibody, 25μg

CD193, also known as CC-chemokine receptor 3 (CCR3), CC CKR3, MIP1-α receptor like-2, and eotaxin receptor, is a member of the G protein-coupled, seven transmembrane receptor family. It binds to the CC chemokines eotaxin, eotaxin-2, and eotaxin-3 with high affinity. CD193 has also been reported to bind RANTES, MCP-3, and MCP-4 with low affinity. CD193 is expressed on mouse eosinophils, basophils, mast cells, mononuclear phagocytes, platelets, hematopoietic progenitor cells, and keratinocytes. It is thought to play a role in allergic diseases such as bronchial asthma and allergic rhinitis. CD193 also function as a co-receptor for HIV-1 and HIV-2, and the binding of eotaxin with CD193 has been shown to inhibit HIV infection in some cell types.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CCR3-transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Alam A, et al. 2022. Cancer Cell. 40:153. PubMed Blomberg OS 2023. Cancer Cell. 41(1):106-123.e10. PubMed Liu J, et al. 2020. Am J Pathol. 190:1298. PubMed Chen C, et al. 2015. J Exp Med. 212:73. PubMed Ku MW, et al. 2020. Cell Host Microbe. 29(2):236-249.e6. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Yang Y, et al. 2016. Nat Commun. 7:11385. PubMed Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed Siddique SM, et al. 2019. Sci Rep. 9:13977. PubMed Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed Giridhar P, et al. 2016. J Immunol. 197(11):4228-4239. PubMed Dietschmann A, et al. 2020. Eur J Immunol. 50:1044. PubMed
RRID: AB_2561608 (BioLegend Cat. No. 144509) AB_2561609 (BioLegend Cat. No. 144510)
Structure: G-protein coupled, seven transmembrane domain receptor, 356 amino acids
Distribution: Eosinophils, basophils, mast, mononuclear phagocytes, platelets, hematopoietic progenitor, keratinocytes
Function: Co-receptor for HIV-1 and HIV-2, allergy
Ligand/Receptor: Eotaxin, eotaxin-2, eotaxin-3, RANTES, MCP-3, and MCP-4
Cell Type: Basophils, Eosinophils, Hematopoietic stem and progenitors, Mast cells, Platelets
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Zlotnik A, et al. 2006. Genome Biol. 7:243. 2. Kodali RB, et al. 2004. Arterioscler. Thromb. Vasc. Biol. 24:1211. 3. Das AM, et al. 2006. J. Pharmacol. Exp. Ther. 318:411. 4. Huaux F, et al. 2005. Am. J. Pathol. 167:1485. 5. Puxeddu I, et al. 2006. J. Allergy Clin. Immunol. 117:103.
Gene ID: 12771
UniProt: View information about CD193 on UniProt.org
Clone: J073E5
Regulatory Status: RUO
Other Names: CC CKR3, MIP1-α receptor like-2, eotaxin receptor, MIP1-alpha receptor like-2
Isotype: Rat IgG2a, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.