Biolegend, 144517, Brilliant Violet 421™ anti-mouse CD193 (CCR3) Antibody, 50μg

CD193, also known as CC-chemokine receptor 3 (CCR3), CC CKR3, MIP1-α receptor like-2, and eotaxin receptor, is a member of the G protein-coupled, seven transmembrane receptor family. It binds to the CC chemokines eotaxin, eotaxin-2, and eotaxin-3 with high affinity. CD193 has also been reported to bind RANTES, MCP-3, and MCP-4 with low affinity. CD193 is expressed on mouse eosinophils, basophils, mast cells, mononuclear phagocytes, platelets, hematopoietic progenitor cells, and keratinocytes. It is thought to play a role in allergic diseases such as bronchial asthma and allergic rhinitis. CD193 also function as a co-receptor for HIV-1 and HIV-2, and the binding of eotaxin with CD193 has been shown to inhibit HIV infection in some cell types.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CCR3-transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Product Citations: Knuplez E, et al. 2021. British Journal of Pharmacology. 178(5):1234-1248. PubMed
RRID: AB_2565743 (BioLegend Cat. No. 144517)
Structure: G-protein coupled, seven transmembrane domain receptor, 356 amino acids
Distribution: Eosinophils, basophils, mast, mononuclear phagocytes, platelets, hematopoietic progenitor, keratinocytes
Function: Co-receptor for HIV-1 and HIV-2, allergy
Ligand/Receptor: Eotaxin, eotaxin-2, eotaxin-3, RANTES, MCP-3, and MCP-4
Cell Type: Basophils, Eosinophils, Hematopoietic stem and progenitors, Mast cells, Platelets
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Zlotnik A, et al. 2006. Genome Biol. 7:243. 2. Kodali RB, et al. 2004. Arterioscler. Thromb. Vasc. Biol. 24:1211. 3. Das AM, et al. 2006. J. Pharmacol. Exp. Ther. 318:411. 4. Huaux F, et al. 2005. Am. J. Pathol. 167:1485. 5. Puxeddu I, et al. 2006. J. Allergy Clin. Immunol. 117:103.
Gene ID: 12771
UniProt: View information about CD193 on UniProt.org
Clone: J073E5
Regulatory Status: RUO
Other Names: CC CKR3, MIP1-α receptor like-2, eotaxin receptor, MIP1-alpha receptor like-2
Isotype: Rat IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.