Biolegend, 144802, Purified anti-mouse CD124 (IL-4Rα) Antibody, 100μg

CD124, a subunit of IL-4R, is a 140 kD transmembrane glycoprotein. It associates with either the common γ-chain (CD132) to form the type I IL-4R complex, which specifically binds IL-4, or with IL-13Ra1 to form the type II IL-4R heterodimeric complex, which binds and transduces signals from either IL-4 or IL-13.  A truncated form of IL-4Rα exists in soluble form in biological fluids. CD124 is expressed on mouse late precursors and mature B cells, T cells, monocytes, and has low expression on granulocytes.  In B cells, CD124 can bind IL-4 and IL-13 and regulate IgE antibody production. In T cells, the type I IL-4R (IL-4R/gC) is mostly responsible for Th2 cell expansion by mediating IL-4-dependent activation of the transcription factors in hematopoietic cells. The type II IL-4R (IL-4R/IL-13Ra1) is the main route for nonhematopoietic cell responses to IL-4 or IL-13.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Mouse IL4Rα encoding the extracellular domain (amino acid residues 26 - 233) was fused to the carboxy-terminal Fc region of human IgG1 via a linker peptide.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Gubin MM, et al. 2018. Cell. 175:1014. PubMed Maier B, et al. 2020. Nature. 580:257. PubMed
RRID: AB_2561554 (BioLegend Cat. No. 144801) AB_2561555 (BioLegend Cat. No. 144802)
Structure: 140 kD type I transmembrane glycoprotein, associates with common γ chain or IL-13 receptor alpha-1 subunit
Distribution: B cells, T cells, monocytes, and low levels on granulocytes.
Function: Regulates IgE antibody production in B cells, Th2 cell expansion, and IL-4 or IL-13 induced response in non-hematopoietic cells
Interaction: STAT6
Ligand/Receptor: IL-4, IL-13
Cell Type: B cells, Granulocytes, Monocytes, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Kashiwada M, et al. 2001. J. Immunol. 167:6382. 2. Gilmour J, et al. 2008. J. Immunol. 124:437. 3. Hage T, et al. 1999. Cell 97:271.
Gene ID: 16190
UniProt: View information about CD124 on UniProt.org
Clone: I015F8
Regulatory Status: RUO
Other Names: IL-4R
Isotype: Rat IgG2b, κ