Biolegend, 145301, Purified anti-mouse IL-33Rα (IL1RL1, ST2) Antibody, 25μg

IL-33Rα, also known as ST2 or IL-1RL1, is a member of the Toll/IL-1 receptor family.  It binds IL-33 and is structurally similar to IL-1R1.  Two forms of the protein exist - a soluble form known as ST2 and a membrane anchored form known as ST2L.  The membrane form is expressed by Th2 cells and bone marrow derived mast cells, whereas the soluble form is expressed by serum-stimulated fibroblasts. Blocking with anti-ST2 antibodies has been shown to alleviate experimental arthritis and airway inflammation.  The IL-33-ST2 axis has been reported to be important across a range of diseases including asthma, allergies, and cardiac disease.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-33Rα-hFcγ1 fusion protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Hashiguchi M, et al. 2014. Eur. J. Immunology. (FC) PubMed
Product Citations: Zheng P, et al. 2023. Stroke Vasc Neurol. . PubMed Malinczak CA, et al. 2021. J Immunol. 206:1315. PubMed Chudnovskiy A et al. 2016. Cell. 167(2):444-456 . PubMed Matsuba S, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01538. PubMed Schuler CF, et al. 2020. Allergy. 75:2279. PubMed Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
RRID: AB_2561842 (BioLegend Cat. No. 145301) AB_2561843 (BioLegend Cat. No. 145302)
Structure: 63 kD, IL-1 receptor family member, single pass type I membrane protein
Distribution: Mast cells, Th2 cells, and fibroblasts
Function: Following interaction with IL-33, it recruits MyD88, Irak1, Irak4 and Traf6 followed by phosphorylation of the MAP/ERK pathway.
Interaction: Interacts with MyD88, Irak1, Irak4 and Traf6
Ligand/Receptor: IL-33
Cell Type: Fibroblasts, Mast cells, Th2
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: 1. Yanagisawa K, et al. 1993. FEBS Lett. 318:83. 2. Schmitt E, et al. 1990. Cytokine 6:407. 3. Yanagisawa K, et al. 1992. FEBS Lett. 302:51. 4. Takagi T, et al. 1993. Biochim Biophys Acta. 1178:194.
Gene ID: 17082
UniProt: View information about IL-33Ralpha on UniProt.org
Clone: DIH9
Regulatory Status: RUO
Other Names: T1/ST2, ST2L, IL-1R1, T1, DER4, IL1R1
Isotype: Rat IgG2a, κ