Biolegend, 145321, Brilliant Violet 785™ anti-mouse IL-33Rα (IL1RL1, ST2) Antibody, 50μg

IL-33Rα, also known as ST2 or IL-1RL1, is a member of the Toll/IL-1 receptor family.  It binds IL-33 and is structurally similar to IL-1R1.  Two forms of the protein exist - a soluble form known as ST2 and a membrane anchored form known as ST2L.  The membrane form is expressed by Th2 cells and bone marrow derived mast cells, whereas the soluble form is expressed by serum-stimulated fibroblasts. Blocking with anti-ST2 antibodies has been shown to alleviate experimental arthritis and airway inflammation.  The IL-33-ST2 axis has been reported to be important across a range of diseases including asthma, allergies, and cardiac disease.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-33Rα-hFcγ1 fusion protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Hashiguchi M, et al. 2014. Eur. J. Immunology. (FC) PubMed
RRID: AB_2860702 (BioLegend Cat. No. 145321)
Structure: 63 kD, IL-1 receptor family member, single pass type I membrane protein
Distribution: Mast cells, Th2 cells, and fibroblasts
Function: Following interaction with IL-33, it recruits MyD88, Irak1, Irak4 and Traf6 followed by phosphorylation of the MAP/ERK pathway.
Interaction: Interacts with MyD88, Irak1, Irak4 and Traf6
Ligand/Receptor: IL-33
Cell Type: Fibroblasts, Mast cells, Th2
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: 1. Yanagisawa K, et al. 1993. FEBS Lett. 318:83. 2. Schmitt E, et al. 1990. Cytokine 6:407. 3. Yanagisawa K, et al. 1992. FEBS Lett. 302:51. 4. Takagi T, et al. 1993. Biochim Biophys Acta. 1178:194.
Gene ID: 17082
UniProt: View information about IL-33Ralpha on UniProt.org
Clone: DIH9
Regulatory Status: RUO
Other Names: T1/ST2, ST2L, IL-1R1, T1, DER4, IL1R1
Isotype: Rat IgG2a, κ