Biolegend, 145521, PE/Dazzle™ 594 anti-mouse CD185 (CXCR5) Antibody, 25μg

CD185 is also known as CXCR5. It is the receptor for chemokine CXCL13/BLC, which is chemotactic for B cells.  CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow.  It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches.  Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: mCXCR5-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.
Product Citations: Hudson WH, et al. 2020. Immunity. 51(6):1043-1058.e4.. PubMed Topchyan P, et al. 2022. Cell Rep. 111736:41. PubMed Moon S, et al. 2024. JCI Insight. :. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed
RRID: AB_2563643 (BioLegend Cat. No. 145521) AB_2563644 (BioLegend Cat. No. 145522)
Structure: 42 kD, seven transmembrane protein
Distribution: B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain
Function: B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma
Ligand/Receptor: BLC/CXCL13
Cell Type: B cells, T cells, Tfh
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532. 2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381. 3. Forster R, et al. 1994. Blood 84:830. 4. Forster R, et al. 1996. Cell 87:1037. 5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831.
Gene ID: 12145
UniProt: View information about CD185 on UniProt.org
Clone: L138D7
Regulatory Status: RUO
Other Names: BLR1, GPCR6, MDR-15
Isotype: Rat IgG2b, κ