Biolegend, 145902, Purified anti-mouse Plexin B2 Antibody, 500μg

Plexin B2 is a 240 kD transmembrane receptor belonging to the semaphorin receptor family, B subfamily. All the family members share an extracellular semaphorin domain and an intracellular plexin domain-containing tail that can mediate intracellular signaling. Distinct from the other subfamily members, Plexin B2 contains an intracellular domain with a PDZ motif (post synaptic density protein (PSD95), Drosophila disc large tumor suppressor (DlgA), and zonula occludens-1 protein (zo-1)), which can relay extracellular signals to intracellular motifs. Similar to the other B family members, Plexin B2 was also originally found in the nervous system and was later reported on endocrine, reproductive, urinary, digestive, respiratory, and immune systems. In immune cells, Plexin B2 is highly expressed on macrophages, conventional dendritic cells (cDCs), and plasmacytoid dendritic cells (pDCs).  It is also expressed on T cells and T-dependent germinal center B cells. Plexin B2 facilitates ligand induced cell guidance and migration in the nervous system, and induces cytoskeletal changes in overexpression assays through RhoGTPase. However, the function of Plexin B2 in the immune system is not conclusive. Plexin B2 has been reported to modulate upon activation of DCs by TLR ligands, TNF-α, and anti-CD40. It also functions as a negative regulator of Rac and Cdc42 and modulates cell motility in macrophages. Plexin B2 has been found to have several semaphorin ligands including Semaphorin-3E, Semaphorin-4A, Semaphorin-4C, and Semaphorin-4D.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: PAM 2-12 keratinocyets cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Cruz JW, et al. 2019. Sci Rep. 9:12031. PubMed
RRID: AB_2562091 (BioLegend Cat. No. 145901)
Structure: Contains an extracellular semaphorin domain and an intracellular plexin domain-containing tail; intracellular domain has a PDZ motif (PSD95, DlgA, and zo-1)
Distribution: Macrophages, dendritic cells, T cells, T-dependent germinal center B cells
Function: Involved in cell motility, modulate dendritic cells
Ligand/Receptor: Semaphorin-3E, Semaphorin-4A, Semaphorin-4C, and Semaphorin-4D
Cell Type: B cells, Dendritic cells, Macrophages, T cells
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, Semaphorin Receptors
Antigen References: 1. Roney K, et al. 2011. PLoS One 6:e24795. 2. Worzfeld T, et al. 2004. Eur. J. Neurosci. 19:2622. 3. Raper JA, et al. 2000. Curr. Opin. Neurobiol. 10:88. 4. Artigiani S, et al. 2003. J. Bio. Chem. 278:10094.
Gene ID: 140570
UniProt: View information about Plexin B2 on UniProt.org
Clone: 3E7
Regulatory Status: RUO
Isotype: Armenian Hamster IgG