Biolegend, 146010, PerCP/Cyanine5.5 anti-Asialo-GM1 Antibody, 100μg

GM1 is a ganglioside, a type of glycosphingolipid with a single sialic acid group. Asialo-GM1 is a GM1 derivative without a sialic acid group. It is expressed on NK cells, basophils, monocytes/macrophages, and T cells. It is particularly expressed on very early thymocytes, but the expression decreases as the cells mature and become Thy-1 + . The highest expression is detected on neuronal tissues. This molecule has been shown to be involved in microbial pathogenesis. Antibodies specific for Asialo-GM1 are elevated in dementia, lupus, and Guillain-Barré syndrome.
100μg
Verified Reactivity: Mouse, Rat
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Asialo-GM1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: This antibody recognizes asialo-GM1. It does not react with other glycolipids, such as GM1 and Asialo-GM2.This antibody can partially block IL-12 induced IFN-γ production but does not affect other systemic action of IL-12. This product may contain other non-IgG subtypes.
Application References(PubMed link indicates BioLegend citation): Naiki M, et al. 1974. J. Immunol. 113:84. Kasai M, et al. 1981. Nature. 291:334. (Depletion) Nishikado H, et al. 2011. J. Immunol. 186:5766. (Depletion) Zhou G, et al. 2013. Eur. J. Immunol. 43:929. (Depletion)
RRID: AB_2728173 (BioLegend Cat. No. 146010)
Structure: Asialo-GM1 is a GM1 derivative without a sialic acid group, with the structure: βDGalp(1-3)βDGalpNAc(1-4)βDGalp(1-4)βDGlcp(1-1)Cer
Distribution: NK cells, basophils, splenic T lymphocytes, highest concentrations on neurons
Function: Supports cellular identification, neuronal plasticity, and repair mechanisms
Interaction: Facilitates cellular recognition and cell-to-cell communication
Ligand/Receptor: Ligand for cholera toxin
Cell Type: Basophils, Neurons, NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References: 1. Stein-Douglas K, et al. 1979. J. Exp. Med. 143:822. 2. Kasai M, et al. 1980. Eur. J. Immunol. 10:175. 3. Young WW Jr, et al. 1980. J. Immunol. 124:199. 4. Bansal AS, et al. 1994. J. Clin. Path. 47:300.
Gene ID: NA
Clone: Poly21460
Regulatory Status: RUO
Other Names: Ganglioside, GM1, ganglio-N-tetraosylceramide
Isotype: Rabbit Polyclonal IgG
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.