Biolegend, 146506, PE anti-mouse CD184 (CXCR4) Antibody, 100μg

CD184, also known as CXCR4, is a member of the G protein coupled receptor family that binds CXCL12 (SDF1). CXCR4 and CXCL12 play an important role in immune and inflammatory responses through the regulation of cell migration and growth. CXCR4 plays a crucial role in the pathogenesis of several autoimmune diseases such as atherosclerosis, rheumatoid arthritis, and wound healing. In addition, CXCR4 is the cofactor for fusion and entry of the T cell-tropic form of HIV-1.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CXCR4-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vivo blocking1
Application References(PubMed link indicates BioLegend citation): Costa MJ, et al. 2018. PLoS One. 13:e0194688 (Block) PubMed
Product Citations: Abdelwahab T, et al. 2023. iScience. 26:106698. PubMed Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed Regev O, et al. 2022. Front Immunol. 13:849701. PubMed Lin YN, et al. 2022. Oncoimmunology. 11:2027136. PubMed Favre J, et al. 2015. Vascul Pharmacol. Available online 5 August 2015. PubMed Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed Kim Y, et al. 2015. PLoS One. 10:120294. PubMed Nimmagadda SC, et al. 2019. Haematologica. 104:288. PubMed Lee JL, et al. 2022. Aging Cell. 21:e13692. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Mok KW et al. 2018. Developmental cell. 48(1):32-48 . PubMed Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed Li Z, et al. 2018. Front Immunol. 9:347. PubMed Saito–Reis CA, et al. 2018. Mol Biol Cell. 29:2946. PubMed Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed Di L, et al. 2022. BMC Biol. 20:213. PubMed Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed Korin B, et al. 2020. Sleep. :43. PubMed Ramakrishnan R, et al. 2020. Cell Reports. 31(8):107684. PubMed Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
RRID: AB_2562782 (BioLegend Cat. No. 146505) AB_2562783 (BioLegend Cat. No. 146506)
Structure: G-protein-coupled seven transmembrane receptor, 39.7 kD
Distribution: T lymphocytes, monocytes, macrophages, tissue-committed stem/progenitor cells (TCSCs), mast cells, vascular smooth muscle cells (VSMCs)
Function: Development of cardiovascular and central nervous system, hematopoiesis, and colonization of BM by fetal liver-derived hematopoietic stem cells (HSCs) during embryogenesis, homing and egress of CD34+ CXCR4+ progenitor cells from bone marrow, and their migration into peripheral tissues. Critical role in homing of cancer cells to specific metastatic sites. Possible role in the ubiquitin proteosome system.
Interaction: Robo -1
Ligand/Receptor: CXCL12 (SDF-1)
Cell Type: Macrophages, Mast cells, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells
Biology Area: Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Ubiquitin/Protein Degradation
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Kucia M, et al. 2005. Stem Cells 23:879. 2. Muller A, et al. 2001. Nature 410:50. 3. Saini V, et al. 2010. J. Biol. Chem. 285:15566. 4. Prasad A, et al. 2007. J. Leuko. Biol. 82:465. 5. De Klerck B, et al. 2005. Arthritis Res. Ther. 7:R1208. 6. Rueda P, et al. 2008. PLoS One 3:e2543. 7. Feng Y, et al. 1996. Science 272:872.
Gene ID: 12767
UniProt: View information about CD184 on UniProt.org
Clone: L276F12
Regulatory Status: RUO
Other Names: Fusin, LESTR, Sdf1r, Cmkar4, PB-CKR, PBSF/SDF-1
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.