Biolegend, 146508, APC anti-mouse CD184 (CXCR4) Antibody, 100μg

CD184, also known as CXCR4, is a member of the G protein coupled receptor family that binds CXCL12 (SDF1). CXCR4 and CXCL12 play an important role in immune and inflammatory responses through the regulation of cell migration and growth. CXCR4 plays a crucial role in the pathogenesis of several autoimmune diseases such as atherosclerosis, rheumatoid arthritis, and wound healing. In addition, CXCR4 is the cofactor for fusion and entry of the T cell-tropic form of HIV-1.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CXCR4-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vivo blocking1
Application References(PubMed link indicates BioLegend citation): Costa MJ, et al. 2018. PLoS One. 13:e0194688 (Block) PubMed
Product Citations: Alghamri MS, et al. 2022. ACS Nano. 16:8729. PubMed Valadares DG, et al. 2023. iScience. 26:106163. PubMed Pioli KT, et al. 2023. iScience. 26:106223. PubMed Mulcrone PL, et al. 2023. Sci Rep. 13:8436. PubMed Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed Feng X, et al. 2023. Nat Commun. 14:3208. PubMed Wu Y, et al. 2021. Immunity. 54:2595. PubMed Fu JF, et al. 2022. Neoplasia. 29:100802. PubMed Ives A, et al. 2021. FASEB J. 35:e21418. PubMed Li X, et al. 2022. Cell. 185:1709. PubMed Gawish R, et al. 2022. Elife. 11:. PubMed Brooks JF, et al. 2021. Cell Reports. 36(9):109645. PubMed Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed Lutes LK, et al. 2021. eLife. 10:00. PubMed Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed Lin C, et al. 2022. Nat Commun. 13:6869. PubMed Kinoshita M, et al. 2020. Cell Stem Cell. . PubMed Lind L, et al. 2021. J Neurovirol. 27:145. PubMed Dudeck J, et al. 2021. Immunity. 54(3):468-483.e5. PubMed Ulaganathan VK, et al. 2020. Sci Rep. 10:8453. PubMed Tanegashima K et al. 2017. EBioMedicine. 24:247-256 . PubMed
RRID: AB_2562784 (BioLegend Cat. No. 146507) AB_2562785 (BioLegend Cat. No. 146508)
Structure: G-protein-coupled seven transmembrane receptor, 39.7 kD
Distribution: T lymphocytes, monocytes, macrophages, tissue-committed stem/progenitor cells (TCSCs), mast cells, vascular smooth muscle cells (VSMCs)
Function: Development of cardiovascular and central nervous system, hematopoiesis, and colonization of BM by fetal liver-derived hematopoietic stem cells (HSCs) during embryogenesis, homing and egress of CD34+ CXCR4+ progenitor cells from bone marrow, and their migration into peripheral tissues. Critical role in homing of cancer cells to specific metastatic sites. Possible role in the ubiquitin proteosome system.
Interaction: Robo -1
Ligand/Receptor: CXCL12 (SDF-1)
Cell Type: Macrophages, Mast cells, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells
Biology Area: Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Ubiquitin/Protein Degradation
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Kucia M, et al. 2005. Stem Cells 23:879. 2. Muller A, et al. 2001. Nature 410:50. 3. Saini V, et al. 2010. J. Biol. Chem. 285:15566. 4. Prasad A, et al. 2007. J. Leuko. Biol. 82:465. 5. De Klerck B, et al. 2005. Arthritis Res. Ther. 7:R1208. 6. Rueda P, et al. 2008. PLoS One 3:e2543. 7. Feng Y, et al. 1996. Science 272:872.
Gene ID: 12767
UniProt: View information about CD184 on UniProt.org
Clone: L276F12
Regulatory Status: RUO
Other Names: Fusin, LESTR, Sdf1r, Cmkar4, PB-CKR, PBSF/SDF-1
Isotype: Rat IgG2b, κ