Biolegend, 146816, PE/Dazzle™ 594 anti-mouse CD301b (MGL2) Antibody, 100μg

Mouse CD301, also known as macrophage galactose-type C-type lectin, has two homologue genes, CD301a (MGL1) and CD301b (MGL2), while there is only one MGL in human and rat.  Mouse CD301a and CD301b are ~42 kD type II transmembrane glycoproteins containing a cytoplasmic domain, a transmembrane domain, a neck domain, and a carbohydrate recognition domain (CRD) within each molecule. CD301a is mainly expressed on a subset of macrophages and immature dendritic cells (DCs). CD301b is mainly found on conventional DCs. Although CD301a and CD301b share high amino acid sequence homology (92% for the intact sequence and 80% for the CRD), they display different carbohydrate specificities. CD301a was found to be highly specific for Lewis X and Lewis A structures, whereas CD301b, more similar to human MGL, recognizes N-actetylgalactosamine (GalNAc) and galactose, including the O-linked Tn-antigen, TF-antigen, and core 2.  So far, CD301a has been reported to be involved in recognition and  endocytosis of glycoproteins with terminal Gal/GalNAc moieties. This contributes to defense against tumor cell metastasis, tissue remodeling, and clearance of apoptotic cells in embryos. CD301b is involved in the internalization of soluble polyacrylamide polymers (PAA) with α-GalNAc residues (GalNAc-PAA) in bone marrow derived DCs.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Purified and recombinant mouse MGL2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Tatsumi N, et al. 2022. STAR Protoc. 3:101845. PubMed Sil P, et al. 2019. J Allergy Clin Immunol. :. PubMed Utz SG, et al. 2020. Cell. 181(3):557-573. PubMed
RRID: AB_2566026 (BioLegend Cat. No. 146815) AB_2566027 (BioLegend Cat. No. 146816)
Structure: 42 kD type II transmembrane glycoproteins, contains a cytoplasmic domain, a transmembrane domain, a neck domain, and a carbohydrate recognition domain (CRD) within each molecule
Distribution: Dermal dendritic cells
Function: Cell adhesion, cell-cell signaling, glycoprotein turnover, inflammation, tissue remodeling
Interaction: Carbohydrate determinants, GP envelope glycoprotein on Marburg and Ebola viruses
Cell Type: Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Denda-Nagai K, et al. 2010. J. Biol. Chem. 285:19193. 2. Westcott D, et al. 2009. J. Exp. Med. 206:3143. 3. Singh SK, et al. 2009. Mol. Immunol. 46:1240. 4. Sakakura M, et al. 2008. J. Biol. Chem. 283:33665. 5. Tsuiji M, et al. 2002. J. Biol. Chem. 277:28892.
Gene ID: 216864
UniProt: View information about CD301b on UniProt.org
Clone: URA-1
Regulatory Status: RUO
Other Names: MGL, M-ASGP-BP, Macrophage galactose-type C-type lectin
Isotype: Rat IgG2a, λ